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Updated: Jan 29, 2026

Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence
Published on: December 11, 2012
Comparative Analysis of the Gut Bacterial Community in Laboratory-Reared and Seasonally Field-Released Larvae of the
Peng Hou1, Li Liu1, Ding Yang2
1College of Life Science and Engineering, Shenyang University, Shenyang 110044, China.
Abstract:
Analyzing the composition and structure of the gut bacterial community in Antheraea pernyi is essential for improving its economic traits, as well as for understanding gut bacteria-host interactions in lepidopteran insects. This study utilized the Illumina MiSeq PE 300 platform to conduct 16S rRNA gene sequencing for a comparative analysis of gut bacterial community in laboratory-reared and field-released (spring and autumn) Antheraea pernyi larvae of the same strain. The study revealed the specific effects of rearing environment and seasonal variation on the structural and functional dynamics of the larval gut bacterial communities. The composition of the dominant gut bacteria varied significantly with rearing environment and season. Laboratory-reared and spring field-released groups exhibited similar bacterial community structures, whereas the autumn field-released group showed a significant trend toward specialization, characterized by enrichment of specific bacterial taxa. Linear discriminant analysis effect size identified statistically significant biomarkers across samples. Taxonomic analysis revealed that Actinomycetota, Actinobacteria, Mycobacteriales, Dietziaceae, and Dietzia were characteristic of the gut bacteria profile in spring field-released, Lactobacillales, Enterococcaceae, and Enterococcus were enriched in the autumn field-released group, and the laboratory-reared group exhibited a relative dominance of Alphaproteobacteria. Functional prediction indicated that gut bacterial community structure likely influences its metabolic potential, which may suggest an adaptive response of the Antheraea pernyi to distinct ecological environments. This study provides important insights into the highly complex nature of insect-microbe interactions.
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