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UV-C Irradiation Effectiveness on Mpox-Virus-Contaminated Surfaces.
Anna Gidari1, Samuele Sabbatini2, Carlo Pallotto1
1Department of Medicine and Surgery, Clinic of Infectious Diseases, "Santa Maria della Misericordia" Hospital, University of Perugia, 06132 Perugia, Italy.
Low-dose UV-C irradiation effectively inactivates Mpox virus (MpoxV) on common fomite surfaces. This disinfection method shows promise for preventing indirect transmission of MpoxV in various settings.
Area of Science:
- Virology
- Environmental Science
- Infectious Disease Control
Background:
- Mpox virus (MpoxV) outbreaks are increasing globally, raising concerns about transmission routes.
- While close contact is primary, fomite transmission of MpoxV is suspected.
- Effective disinfection strategies are needed to control MpoxV spread.
Purpose of the Study:
- To evaluate the efficacy of UV-C irradiation for inactivating MpoxV on contaminated surfaces.
- To determine the optimal UV-C dosage for MpoxV disinfection.
Main Methods:
- UV-C (254 nm) irradiation was applied to MpoxV on plastic, glass, and stainless steel.
- Viral titers were quantified using TCID50 and plaque assays post-exposure.
- Increasing UV-C doses were tested to determine virucidal activity.
Main Results:
- A UV-C dose of 6.34 mJ/cm² significantly reduced MpoxV load (>2-log reduction) on all tested surfaces.
- Effective inactivation doses (EC90) varied by material: 3.33 mJ/cm² (plastic), 0.81 mJ/cm² (stainless steel), 1.98 mJ/cm² (glass).
- No viable virus was detected on plastic and stainless steel at effective doses.
Conclusions:
- UV-C irradiation is a highly effective method for inactivating MpoxV on fomites.
- Low UV-C doses can rapidly disinfect surfaces, supporting environmental control strategies.
- UV-C disinfection can help prevent indirect MpoxV transmission in healthcare and community settings.
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