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Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
Published on: February 8, 2017
Local cargo delivery to double bilayer compartments
Rui Liu1, Ruslan Ryskulov1, Esteban Pedrueza-Villalmanzo1
1Department of Chemistry and Chemical Engineering, Chalmers University of Technology, 41296, Göteborg, Sweden. aldo.jesorka@gomod.eu.
None:
In this work we investigated the encapsulation of fluorescent cargo during temperature-induced formation of lipid double bilayer membrane pockets on silicon carbide substrates. This exotic mode of autonomous membrane transformation, so far only observed on silicon carbide, leads to double-bilayer-enveloped compartments. For localized superfusion and investigation of cargo transfer to the emerging pockets, a hard polymer (photo-crosslinked SUEX™ epoxy) open space microfluidic device was developed. Encapsulation occurs rapidly, and cargo is effectively retained in pockets between distal and proximal bilayer, but escapes de-wetted compartments through double bilayer ruptures that connect the compartment interior to the surrounding medium.
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