Adsorption of Blood Creatinine on a Heparin-Functionalized Surface of Copper-Based Metal-Organic Frameworks

Area of Science:

• Materials Science
• Biomedical Engineering
• Nanotechnology

Background:

• Creatinine accumulation is a major concern in chronic kidney disease (CKD).
• Current treatments for CKD often involve complex procedures with potential complications.
• Developing efficient and safe adsorbents is crucial for improving blood purification.

Purpose of the Study:

• To develop heparin-functionalized copper-based metal-organic frameworks (CuMOFs-Hep) for enhanced creatinine removal.
• To evaluate the hemocompatibility and biosafety of the developed CuMOFs-Hep.
• To assess the potential of CuMOFs-Hep as an adsorbent for blood purification applications.

Main Methods:

• Copper-based metal-organic frameworks (CuMOFs) were synthesized and functionalized with heparin.
• Characterization of CuMOFs-Hep was performed using techniques such as XRD, SEM, FTIR, XPS, and zeta potential analysis.
• Creatinine adsorption capacity and kinetics were evaluated in simulated CKD serum and patient blood samples.
• Biosafety was assessed through copper ion leaching studies and platelet activation assays.

Main Results:

• Heparinization significantly increased the creatinine adsorption capacity of CuMOFs to 267.8 mg/g.
• Rapid creatinine clearance was observed, with 65.9% removal within 30 minutes and 75.0% within 2 hours.
• In patient blood, CuMOFs-Hep reduced creatinine levels by 80.9% (from 854.2 to 163.2 μmol/L).
• Copper ion leaching was significantly reduced by 89.9%, and platelet adhesion/activation was decreased, indicating improved hemocompatibility.

Conclusions:

• CuMOFs-Hep demonstrates efficient and rapid creatinine adsorption in clinically relevant conditions.
• The developed material exhibits low copper leaching and enhanced hemocompatibility.
• CuMOFs-Hep shows significant potential as an effective adsorbent for blood purification in CKD patients.