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A widespread extended arbitrium system controls lysis/lysogeny through antirepression.
Stav Kabel1, Shira Omer Bendori1, Tom Borenstein1
1Shmunis School of Biomedicine and Cancer Research, Faculty of Life Sciences, Tel Aviv University, 6139001 Tel Aviv, Israel.
Many Bacillus phages use the arbitrium system for lysis-lysogeny decisions. A newly identified antirepressor protein, AimX, binds the phage repressor AimC, controlling lysis activation and phage life cycle strategies.
Area of Science:
- Microbiology
- Molecular Biology
- Virology
Background:
- Temperate Bacillus phages utilize the arbitrium system for regulating lysis-lysogeny decisions.
- The secreted peptide AimP inhibits the AimR receptor to promote lysogeny, but downstream mechanisms remain unclear.
Purpose of the Study:
- To elucidate the downstream mechanism of AimR-mediated lysis control in Bacillus phages.
- To identify the function of the extended five-gene module in arbitrium systems.
Main Methods:
- Bioinformatic analysis to identify conserved gene modules.
- Experimental validation across multiple phages.
- Structural characterization of protein interactions.
Main Results:
- Identified a conserved five-gene module in ~75% of arbitrium systems, including aimX, aimC, and aimL.
- AimX, a small antirepressor protein, inhibits AimC repressor function by preventing oligomerization and DNA binding.
- This mechanism activates the pro-lytic aimL gene and other lytic genes, as validated structurally and across phages.
Conclusions:
- Elucidated the predominant molecular strategy for phage lysis-lysogeny control in arbitrium systems.
- Highlighted the crucial role of small antirepressor proteins like AimX in phage decision-making processes.
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