Framework Doping Enhances COF-DNA Nanoplatforms for Dual RNA Imaging and Phototherapy
Peng Gao1, Mengyao Yu1, Ruyue Wei1
1College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, P. R. China.
This study introduces a new method for engineering DNA-based covalent organic frameworks (COFs) for cancer theranostics. The strategy optimizes both imaging and therapy, enabling precise cancer cell detection and destruction.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Chemical Engineering
Background:
- DNA-engineered covalent organic frameworks (COFs) show potential for cancer theranostics.
- Controlling COF-DNA interactions is vital for theranostic performance but remains challenging.
Purpose of the Study:
- To develop a framework doping strategy for optimizing COF-DNA nanosystems.
- To enhance simultaneous diagnostic imaging and phototherapeutic effects.
Main Methods:
- In situ doping of porphyrin into COF nanoparticles.
- Utilizing COFs for photocatalytic reactive oxygen species (ROS) generation.
- Adsorbing and quenching fluorescent single-stranded DNA (ssDNA).
Main Results:
- Achieved simultaneous optimization of diagnostic imaging and phototherapy.
- Constructed a COF-DNA nanosystem for RNA-imaging guided phototherapy.
- System restored fluorescence for cancer-associated RNA detection and generated ROS for cell killing upon laser irradiation.
Conclusions:
- The framework doping strategy effectively regulates COF-DNA interactions.
- Developed a high-performance nanosystem for integrated cancer imaging and phototherapy.
- Provides insights for designing advanced theranostic platforms.
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