Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imprinting01:22

Imprinting

11.1K
Behavioral imprinting is observed in some newborn animals and occurs when they develop strong and specific attachments to another animal (usually a parent) following brief, early-life exposures. Offspring imprint onto parents within a brief period after birth or hatching; this time window is called the critical period. Once imprinting occurs, the bond established between the parents and their offspring is usually long-lasting.
11.1K
Protein Complex Assembly02:41

Protein Complex Assembly

16.8K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.8K
Protein Complex Assembly02:41

Protein Complex Assembly

2.6K
2.6K
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

37.2K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.2K
Real Time RT-PCR02:57

Real Time RT-PCR

65.1K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
65.1K
What are Viruses?00:50

What are Viruses?

128.2K
Overview
128.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A lightweight detector with hybrid pooling and checkerboard attention for solar panel anomalies.

iScience·2026
Same author

TeaWeeding-Action: a vision-based dataset for weeding behavior recognition in tea plantations.

Frontiers in plant science·2026
Same author

Organic Radicals for Tumor Therapy.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Smart pyraclostrobin nanopesticides based on chitosan- and pectin-gated metal-organic framework PCN-777 for boosted efficacy and biosafety.

International journal of biological macromolecules·2025
Same author

Ferroptosis based on metal-organic frameworks for tumor therapy.

Chemical communications (Cambridge, England)·2025
Same author

[Risk assessment of residual dizziness after repositioning in patients with benign paroxysmal positional vertigo according on multivariate analysis and nomogram].

Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery·2025

Related Experiment Video

Updated: Feb 3, 2026

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
14:18

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Published on: October 4, 2011

14.9K

Protein/virus-imprinted self-assembled monolayers for real-time selective detection.

Wanlan Yang1, Ru Han1, Xuezhong Du1

  • 1State Key Laboratory of Coordination Chemistry, MOE Key Laboratory of Mesoscopic Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Advances in Colloid and Interface Science
|February 1, 2026
PubMed
Summary

This study reviews protein/virus-imprinted self-assembled monolayers (SAMs) fabricated in water. This technique creates specific cavities for biomolecules, enabling accurate sensor development without harsh chemicals.

Keywords:
ImprintingProteinsSelf-assembled monolayersSensorsViruses

More Related Videos

Cell-Based Electrical Impedance Platform to Evaluate Zika Virus Inhibitors in Real Time
05:49

Cell-Based Electrical Impedance Platform to Evaluate Zika Virus Inhibitors in Real Time

Published on: March 24, 2023

1.2K
Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host
07:23

Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host

Published on: September 14, 2021

2.7K

Related Experiment Videos

Last Updated: Feb 3, 2026

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
14:18

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Published on: October 4, 2011

14.9K
Cell-Based Electrical Impedance Platform to Evaluate Zika Virus Inhibitors in Real Time
05:49

Cell-Based Electrical Impedance Platform to Evaluate Zika Virus Inhibitors in Real Time

Published on: March 24, 2023

1.2K
Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host
07:23

Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host

Published on: September 14, 2021

2.7K

Area of Science:

  • Materials Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Protein and virus imprinting is a significant challenge in biomaterial development.
  • Existing methods often involve harsh conditions that can denature biomolecules.

Purpose of the Study:

  • To review the fabrication and advantages of protein/virus-imprinted self-assembled monolayers (SAMs) in aqueous media.
  • To highlight the potential of this technique for creating advanced biomacromolecule sensors.

Main Methods:

  • Fabrication of SAMs on gold surfaces using alkanethiols and protein/virus templates in aqueous solution.
  • Formation of complementary cavities via non-covalent interactions and dynamic covalent bonds, utilizing gold surface roughness.
  • Utilizing AuS bonds for self-assembly without polymerization initiators.

Main Results:

  • Developed protein/virus-imprinted SAMs in aqueous media, avoiding organic solvents and polymerization initiators.
  • Created selective binding sites within SAMs through complementary cavities, enabling rapid biomolecule interaction.
  • Demonstrated potentiometric imprinted SAM sensors with accuracy comparable to enzyme-linked immunosorbent assay (ELISA).

Conclusions:

  • Protein/virus-imprinted SAMs offer a versatile platform for fabricating biomacromolecule sensors with high accuracy.
  • The technique avoids biomolecule denaturation and allows for direct integration with transducer surfaces for real-time monitoring.
  • Future work should focus on optimizing thiol selection and surface roughness engineering for enhanced sensor performance.