Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

16.1K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
16.1K
Antibody Structure01:10

Antibody Structure

65.6K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.6K
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

14.5K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
14.5K
Membrane Domains01:18

Membrane Domains

7.2K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
7.2K
Three Developmental Domains01:29

Three Developmental Domains

1.1K
Human development is typically examined across three main domains: physical, cognitive, and socio-emotional. These domains represent the significant areas of change and continuity throughout the lifespan, from infancy to late adulthood.
Physical Development
Physical processes, also known as maturation, encompass the biological changes that occur across an individual's life. These changes begin with genetic inheritance and continue through various stages, including growth in height and weight,...
1.1K
Three-Domain System of Life01:21

Three-Domain System of Life

1.1K
Ribosomal RNA (rRNA) sequence analysis revealed three distinct groups of cells: eukaryotes, bacteria, and archaea. In 1978, Carl R. Woese proposed the concept of domains, a taxonomic level above kingdoms, to differentiate these groups. He suggested that archaea and bacteria, despite their similar appearance, represent separate domains. Domains differ in rRNA, membrane lipid structure, transfer RNA, and antibiotic sensitivity.In this classification, animals, plants, and fungi belong to the...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Baroreceptor Mechanotransduction: Diverse Sensors, Unified Signals.

Hypertension (Dallas, Tex. : 1979)·2026
Same author

Soluble CD146 reflects altered endothelial and metabolic homeostasis in peripheral artery disease.

Angiogenesis·2026
Same author

CD146 extracellular vesicles accumulate at atherosclerotic lesions, reducing inflammation and atheroma burden.

Atherosclerosis·2026
Same author

Extracellular vesicles in atherosclerotic cardiovascular disease: mechanisms and therapeutic implications.

European heart journal·2026
Same author

Fluorinated Amphiphilic Dendrimer to Improve PET Imaging of Cancer.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

The road to Paris: ISTH 2026 Congress sets to debut the latest science and innovation.

Journal of thrombosis and haemostasis : JTH·2026

Related Experiment Video

Updated: Feb 4, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

19.2K

Selection and optimization strategy for Rap1-targeting single-domain antibodies as platelet activation markers.

Marie-Christine Alessi1, Maxime Moulard2, Daniele Boulay-Moine2

  • 1Aix Marseille University, C2VN, INSERM, INRAE, Marseille, France.

Research and Practice in Thrombosis and Haemostasis
|February 2, 2026
PubMed
Summary

Researchers developed novel minibodies to detect active Rap1 in platelets, crucial for platelet activation. A new ELISA test enables monitoring Rap1 activation in clinical studies.

Keywords:
GTPRap1antibodieshumanplatelet activation

More Related Videos

Immunization of Alpacas Lama pacos with Protein Antigens and Production of Antigen-specific Single Domain Antibodies
05:27

Immunization of Alpacas Lama pacos with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

Published on: January 26, 2019

24.6K
Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
04:37

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

1.1K

Related Experiment Videos

Last Updated: Feb 4, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
12:55

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

19.2K
Immunization of Alpacas Lama pacos with Protein Antigens and Production of Antigen-specific Single Domain Antibodies
05:27

Immunization of Alpacas Lama pacos with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

Published on: January 26, 2019

24.6K
Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
04:37

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

1.1K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Rap1 is a key regulator of platelet activation pathways.
  • Understanding Rap1's role is vital for platelet function research.

Purpose of the Study:

  • To develop VHH-Fc minibodies for quantifying active Rap1 levels in platelets.
  • To create tools for studying Rap1-mediated platelet activation.

Main Methods:

  • Phage display library screening for VHHs against active Rap1.
  • Random mutagenesis and yeast 2-hybrid screening for VHH optimization.
  • Subcloning and characterization of VHH-Fc constructs.

Main Results:

  • Generated VHH-Fc clones that selectively detect active Rap1B G12V.
  • Successfully captured active Rap1 from platelet lysates and stimulated platelets.
  • Optimized VHH-Fc B89 and B14 showed high affinity and specificity for active Rap1.
  • Developed an ELISA for detecting active Rap1 in platelets.

Conclusions:

  • Developed VHH-Fc minibodies are effective tools for capturing active Rap1 in platelets.
  • The novel ELISA test facilitates monitoring of platelet Rap1 activation in clinical settings.