Single-cell RNA-seq data of wild type and fli1b mutant zebrafish embryos

Area of Science:

• Developmental Biology
• Genomics
• Zebrafish Models

Background:

• Fli1b is an ETS transcription factor crucial for zebrafish vascular and hematopoietic development.
• Previous studies have implicated Fli1b in these critical developmental processes.

Purpose of the Study:

• To generate and analyze single-cell RNA sequencing data from wild-type and maternal zygotic fli1b mutant zebrafish embryos.
• To identify cell clusters and gene expression patterns affected by the absence of Fli1b.

Main Methods:

• Single-cell suspensions were prepared from approximately 40 wild-type and maternal-zygotic (MZ) fli1b mutant zebrafish embryos at 24 hours post-fertilization.
• RNA sequencing was performed using the 10X Genomics Chromium platform.
• Bioinformatic analysis identified 34 distinct cell clusters, annotated by marker gene expression.

Main Results:

• Integration of wild-type and fli1b mutant datasets revealed 34 distinct cell clusters.
• Cell clusters were annotated based on specific marker gene expression profiles.
• The data provides a comprehensive transcriptomic landscape of early zebrafish development in the context of Fli1b function.

Conclusions:

• The generated single-cell RNA sequencing data is valuable for understanding the molecular mechanisms of vascular and hematopoietic development in zebrafish.
• These transcriptomic datasets can be utilized to investigate broader developmental mechanisms in zebrafish and other model organisms.