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In Situ Analysis of Historical Preservation Fluids in Sealed Containers with Spatially Offset Raman Spectroscopy.

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Monitoring preservation fluids in natural history collections is crucial. Spatially offset Raman spectroscopy (SORS) offers a portable, noninvasive method for identifying these critical conservation fluids.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Heritage Science

Background:

  • Preservation fluids are vital for maintaining the integrity of specimens in natural history collections.
  • Current analytical methods for characterizing these fluids are often invasive or lack portability.
  • Noninvasive, in situ monitoring is needed for effective collection management.

Purpose of the Study:

  • To introduce and evaluate spatially offset Raman spectroscopy (SORS) for in situ classification of preservation fluids.
  • To assess the feasibility of using a portable SORS device for analyzing historical biological specimens.
  • To explore the potential of SORS in understanding fluid-container interactions.

Main Methods:

  • Application of spatially offset Raman spectroscopy (SORS) on 46 fluid-preserved samples.
  • Utilized a portable SORS device for in situ analysis under collection conditions.
  • Employed multivariate analysis for classification of preservation fluid spectra.

Main Results:

  • Accurate identification of preservation fluids in 78.5% of samples.
  • Partial agreement in 15% of cases, particularly for complex or visually similar solutions.
  • Distinguished between glass and plastic containers, offering insights into storage history.

Conclusions:

  • SORS combined with multivariate analysis is a powerful, noninvasive tool for preservation fluid identification.
  • The method demonstrates high chemical specificity and sensitivity to mixtures.
  • This approach has significant potential for conservation and collection management in natural history institutions.