Related Experiment Video
Updated: Feb 4, 2026

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
Published on: July 22, 2014
Small RNA Profiling in Trypanosomes
Florencia Díaz-Viraqué1, Gonzalo Greif1, María Rosa García-Silva1
1Laboratorio de Interacciones Hospedero-Patógeno-UBM, Institut Pasteur de Montevideo, Montevideo, Uruguay.
None:
Trypanosomatids express a diverse repertoire of small non-coding RNAs despite lacking the canonical RNA interference machinery in most species. These small RNAs-including fragments derived from tRNAs, rRNAs, and other non-coding sources-are thought to play regulatory roles in parasite biology and host-parasite interactions. In this chapter, we provide a detailed protocol for the isolation, library preparation, sequencing, and computational analysis of small RNAs in trypanosomes. The method is optimized to preserve short RNA species while minimizing artifacts such as adaptor dimers and concatamerization. It includes quality control and size selection steps to ensure the reproducibility and high quality of the libraries, allowing the study of the transcriptome of small RNAs in these parasites.
More Related Videos
14:26Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers Diethylaminoethyl-cellulose Columns
Published on: April 6, 2019
09:12Calcified Artery Preparation and Processing with Preserved Morphology and RNA for Digital Spatial Profiling
Published on: January 23, 2026
Related Concept Videos
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Structure
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Stability
RNA Splicing
RNA Editing
Eukaryotic RNA Polymerases
All three eukaryotic RNAPs require specific transcription factors, of which the...