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T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
Published on: July 31, 2010
Metabolomics Analysis of Trypanosomes Using Ion Mobility-Enhanced Mass Spectrometry
Andrew MacKenzie1, Fraser Massie1, Tessa Moses2
1EdinOmics, RRID:SCR_021838, Centre for Engineering Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh, UK.
Abstract:
Metabolomics, the comprehensive study of low-molecular-weight metabolites, provides a direct snapshot of an organism's physiological state and complements genomics and proteomics. Ion mobility (IM) enhanced metabolomics adds crucial gas-phase separation, resolving isobaric and isomeric metabolites for deeper biological insights. Here we describe a method coupling rapid liquid chromatography to IM-enhanced mass spectrometry, which allows high-throughput metabolomics analysis. This method was successfully applied to trypanosomes to elucidate the mode of action of a small-molecule inhibitor in Trypanosoma brucei.
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