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Updated: Feb 4, 2026

Immunization of Alpacas Lama pacos with Protein Antigens and Production of Antigen-specific Single Domain Antibodies
Published on: January 26, 2019
Camelid Single-Domain Antibodies as Tools for Novel Target Identification in Kinetoplastid Research
Steven Odongo1,2, Line De Vocht3, Bo-Kyung Jin4
1Department of Biomedical Laboratory Technology and Molecular Biology (BLT), College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda.
Abstract:
Camelid single-domain antibodies (sdAbs), commercially known as Nanobodies™, possess remarkable properties that render them highly suitable as versatile tools for target discovery and product development. Interestingly, despite their successful and broad deployment in life sciences, sdAbs remain heavily underutilized in the field of molecular parasitology. In this chapter, we describe how we have employed an unbiased camelid immunization strategy to discover novel diagnostic biomarkers with sdAbs. This protocol shows the potential of camelid sdAbs as powerful tools for novel target discovery in kinetoplastid research.
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