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Updated: Feb 4, 2026

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids
Published on: April 6, 2022
Optimization of agarose-gelatin double embedding to minimize sectioning artifacts in multicellular spheroids
Hyoyoung Maeng1, Min-Gi Han1, Yoseop Jeon1
1School of Advanced Biotechnology, Konkuk University, Seoul, Republic of Korea.
None:
Processing methods that do not induce shape distortion are essential for the analysis of tissue morphology. Although the use of agarose-gelatin double embedding to reduce shape distortion has been suggested, the effect of matrix concentration has not been addressed. Therefore, combinations of agarose (1-3%) and gelatin (1-10%) were evaluated using multicellular spheroids without extracellular matrix as a model of mechanical fragility. In this study, a blend of 2% agarose and 5% gelatin effectively prevented distortion. This protocol can enhance the morphological analysis of delicate tissues.
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