Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Systematic Sampling Method01:17

Systematic Sampling Method

13.3K
Sampling is a technique to select a portion (or subset) of the larger population and study that portion (the sample) to gain information about the population. Data are the result of sampling from a population. The sampling method ensures that samples are drawn without bias and accurately represent the population. Because measuring the entire population in a study is not practical, researchers use samples to represent the population of interest.
Systematic sampling is one of the simplest methods...
13.3K
Histone Modification02:32

Histone Modification

16.1K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
16.1K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

14.8K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.8K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.5K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.5K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

13.3K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
13.3K
Random and Systematic Errors01:20

Random and Systematic Errors

14.9K
Scientists always try their best to record measurements with the utmost accuracy and precision. However, sometimes errors do occur. These errors can be random or systematic. Random errors are observed due to the inconsistency or fluctuation in the measurement process, or variations in the quantity itself that is being measured. Such errors fluctuate from being greater than or less than the true value in repeated measurements. Consider a scientist measuring the length of an earthworm using a...
14.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcending Structural Dependencies: A Tunable Mass Spectrometry-Driven Machine Learning Framework for Genotoxicity Prediction.

Environmental science & technology·2026
Same author

Decoupling Thermodynamic and Kinetic Controls in Methane Activation on High-Spin Rhenium Centers: The Interplay of Electronic Accumulation and Relativistic Spin-Orbit Coupling.

The journal of physical chemistry letters·2026
Same author

Noncontact Extraction Mass Spectrometry with Bubble Extraction Ionization Facilitates Operando Monitoring Electrochemical Reactions.

Analytical chemistry·2026
Same author

Recent Advances in Antibody-Drug Conjugates and Immunotherapy Combinations for Treatment of Triple-Negative Breast Cancer.

Breast cancer (Dove Medical Press)·2026
Same author

Oxidation of Organic Sulfur at the Air-Water Interface of Microdroplets: A New Way of Atmospheric Particle Precursor Formation.

ACS environmental Au·2026
Same author

Artificial Intelligence-Powered Prediction of Microplastic-Pollutant Adsorption Coefficients Enables Scalable Risk Prioritization in Aquatic Environments.

Environmental science & technology·2026
Same journal

Strain-Level Food Surveillance of <i>Escherichia coli</i> Using a Specific-Nonspecific Hybrid Sensor Array Strategy.

Analytical chemistry·2026
Same journal

A Field-Portable Fe(IV)-Mediated Competitive Quenching Chemiluminescence Platform with a Synchronous Y-Shaped Flow-through Cell for Broad-Spectrum Quantification of Volatile Phenols.

Analytical chemistry·2026
Same journal

Single-Molecule Characterization of CRISPR-Cas12a for Amplification-Free Genetic Testing.

Analytical chemistry·2026
Same journal

Integrated Acoustofluidic Manipulation and Oscillation-Stabilized Magnetic Relaxation Biosensing for <i>Salmonella</i> Detection.

Analytical chemistry·2026
Same journal

A Self-Powered Sensing Platform Based on the Janus Heterostructure for Machine Learning-Assisted Dual-Mode Detection of 17β-Estradiol.

Analytical chemistry·2026
Same journal

Large Language Model-Generated Dietary Metabolite Biomarker Database Drives Deep Annotation of the Human Diet Metabolome.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Feb 4, 2026

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

12.3K

A Highly Sensitive and High-Throughput Quantitative HILIC-MS/MS Method for Systematic Profiling of RNA Modifications.

Cheng Guo1, Xiujuan Hong1, Yiqiu Hu1

  • 1Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.

Analytical Chemistry
|February 2, 2026
PubMed
Summary
This summary is machine-generated.

A new hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method accurately quantifies 35 RNA nucleosides. This high-throughput technique enhances epitranscriptome analysis and identifies potential breast cancer biomarkers.

More Related Videos

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
06:31

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

10.3K
Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

7.1K

Related Experiment Videos

Last Updated: Feb 4, 2026

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

12.3K
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
06:31

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

10.3K
Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

7.1K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • The epitranscriptome, encompassing RNA modifications, plays a crucial role in gene regulation.
  • Accurate quantification of multiple modified nucleosides in RNA is essential for understanding epitranscriptomics but faces analytical challenges.

Purpose of the Study:

  • To develop a sensitive and high-throughput analytical method for quantifying numerous RNA nucleosides.
  • To apply the developed method for analyzing RNA modification profiles in breast cancer.

Main Methods:

  • Established a hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method combined with stable isotope-dilution.
  • Utilized malic acid as a mobile phase additive to improve separation and signal response.
  • Achieved simultaneous analysis of 35 nucleosides within 12.5 minutes with high sensitivity (amol to fmol detection limits).

Main Results:

  • The HILIC-MS/MS method demonstrated improved separation of isomeric and isobaric nucleosides.
  • Enhanced MS signal response by 2.5- to 20.0-fold using malic acid.
  • Successfully analyzed RNA modification profiles in breast cancer, revealing alterations across subtypes.

Conclusions:

  • The developed HILIC-MS/MS method offers sensitive, high-throughput detection of multiple modified nucleosides.
  • This analytical tool is valuable for advancing epitranscriptomic research.
  • The method shows potential for screening nucleosides as biomarkers in clinical settings.