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Updated: Feb 4, 2026
On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System
Published on: July 18, 2025
Listeria monocytogenes detection assay via aptamer-functionalized magnetic bead enrichment coupled with
Han Wang1, Haosong Li1, Dexin Zeng2
1State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China.
Abstract:
Conventional detection methods for Listeria monocytogenes suffer from limitations including lengthy procedures, equipment dependency, and operational complexity, making them unsuitable for rapid on-site detection. Therefore, this study developed an LM-RPA-Cas12a-LFA detection method. Target bacteria were enriched using aptamer magnetic beads, followed by RPA amplification of a 196 bp fragment of the hly gene to activate the Cas12a system, with results displayed on lateral flow strips. The method including bacterial enrichment, DNA extraction, LM-RPA-Cas12a-LFA procedure required 2 h with detection limit of 1 × 10-10 ng/µL and 1.35 CFU/mL in complex food matrices, demonstrating excellent reproducibility and stability. Detection of 16 real food samples showed complete concordance with qPCR validation, accurately identifying 4 positive and 12 negative samples. This method provides a reliable, rapid, and sensitive tool for on-site qualitative detection of Listeria monocytogenes.
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