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Ultrasensitive detection of lead ion in tea samples using a versatile and robust multi-DNAzyme DNA machine mediated
Yubo Peng1, Jianguo Xu2, Baoqiang Chen3
1Engineering Research Center of Bio-process, Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
None:
Lead ion (Pb2+) contamination in tea poses serious threats to food safety. We present a novel detection platform integrating a multifunctional DNAzyme machine with the CRISPR/Cas12a system. Upon target Pb2+ binding, the multi-DNAzyme activates the DNA machine, initiating multiple isothermal cycles that generate a poly-A sequence. This sequence activates Cas12a, triggering trans-cleavage of the hairpin fluorescent probes and significant signal amplification. The self-sustained amplification of DNA machine eliminates the need for any auxiliary probes, greatly simplifying the assay design. This platform achieves excellent sensing perfromances with detection limit of 67.53 pM, wide linear range (0.01-100 nM), satisfied specificity and high recovery rates above 96 % in real tea samples. This integration of DNAzyme recognition with CRISPR/Cas12a signal enhancement provides a rapid, and cost-effective method for Pb2+ detection. This strategy offers practical potential for food safety monitoring and environmental assessment, and future research may expand its applicability to other heavy metal contaminants.
