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Updated: Apr 18, 2026
Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
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Asymmetric RPA-primed hybridization chain reaction enabling one-input-multiple-Cas12a activation for ultrasensitive
Qi Wang1, Yuxuan Zhu1, Baoqiang Chen2
1Anhui Provincial Key Laboratory of Green Carbon Chemistry, Anhui Province Key Laboratory of Pollution Damage and Biological Control for Huaihe River Basin, School of Biological and Food Engineering, Fuyang Normal University, Fuyang, Anhui, 236037, PR China.
A new integrated platform enhances food safety by rapidly detecting pathogens like Salmonella. This innovative method uses a novel CRISPR system for faster, more accurate results, improving public health protection.
Area of Science:
- Food safety and pathogen detection
- Molecular diagnostics
- Biotechnology
Background:
- Conventional methods for pathogen detection are slow, complex, and lack accuracy.
- Accurate and rapid detection of foodborne pathogens like Salmonella is crucial for public health.
Purpose of the Study:
- To develop an integrated, sensitive, and specific platform for detecting foodborne pathogens.
- To overcome limitations of existing detection methods through novel molecular amplification and CRISPR technology.
Main Methods:
- Integrated asymmetric recombinase polymerase amplification (aRPA), hybridization chain reaction (HCR), and CRISPR/Cas12a.
- Designed a single-tube assay enabling cooperative amplification via multiple Cas12a activation.
- Utilized a DNA releaser for simplified sample preparation.
Main Results:
- Achieved a detection limit of 1.8 x 10^2 CFU/mL in direct screening and 5 CFU/mL after enrichment.
- Demonstrated high specificity for common foodborne pathogens.
- Validated reliable performance in spiked food samples.
Conclusions:
- The integrated aRPA-HCR-CRISPR/Cas12a platform offers a sensitive, specific, and practical solution for food safety monitoring.
- This cooperative amplification strategy significantly enhances detection capabilities.
- The developed method provides a reliable tool for rapid foodborne pathogen detection.
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