Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

3.7K
Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
3.7K
RNA-seq03:21

RNA-seq

12.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.1K
Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

1.4K
Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
1.4K
Sampling Methods: Sample Types01:18

Sampling Methods: Sample Types

3.3K
Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
3.3K
Classification of Titrimetric Analysis Based on Reaction Types01:01

Classification of Titrimetric Analysis Based on Reaction Types

1.8K
Titrimetric analysis in solution chemistry involves measuring the volume of solutions and is often called volumetric analysis. The standard solution of known concentration in the burette is called the titrant, whereas the solution of unknown concentration in the flask is called the analyte, or titrand. Titrimetric analyses can be classified into four types based on the reactions between the titrant and analyte.
Titrations between an acid and a base lead to neutralization reactions that form...
1.8K
Tissues01:18

Tissues

85.3K
Cells with similar structure and function are grouped into tissues. A group of tissues with a specialized function is called an organ. There are four main types of tissue in vertebrates: epithelial, connective, muscle, and nervous.
85.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In preprints: toward a holistic lineage-tracing map of mammalian embryogenesis.

Development (Cambridge, England)·2026
Same author

Integrated multi-omic atlas reveals the hierarchy of spatiotemporal regulatory networks of mouse gastrulation.

Nature communications·2026
Same author

Asynchronous mouse embryo polarization leads to heterogeneity in cell fate specification.

eLife·2025
Same author

Posterior enhancer (p-Enh) maintains early neuromesodermal progenitors bi-potency during gastrulation.

Cell regeneration (London, England)·2025
Same author

Asynchronous mouse embryo polarization leads to heterogeneity in cell fate specification.

bioRxiv : the preprint server for biology·2025
Same author

Gastrula-Premarked Posterior Enhancer Primes Posterior Tissue Development Through Cross-Talk with TGF-β Signaling Pathway.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Related Experiment Video

Updated: Feb 4, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

7.6K

Transcriptomic Analysis from Cryosectioned Tissue Samples Using Laser Capture Microdissection-Based Geo-Seq.

Xianfa Yang1,2, Yingying Chen3, Yun Yang3,4

  • 1Guangzhou National Laboratory, Guangzhou International Bio Island, Guangzhou, Guangdong, China. yang_xianfa@gzlab.ac.cn.

Methods in Molecular Biology (Clifton, N.J.)
|February 2, 2026
PubMed
Summary
This summary is machine-generated.

Geographical position sequencing (Geo-seq) maps gene expression in specific tissue locations. This method analyzes transcriptomic architecture, revealing gene abundance and spatial patterns from single tissue samples.

Keywords:
Laser capture microdissectionRNA isolationReverse transcriptionTranscriptomic analysis

More Related Videos

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

4.4K
Laser Capture Microdissection of Mammalian Tissue
16:34

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

17.5K

Related Experiment Videos

Last Updated: Feb 4, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

7.6K
Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

4.4K
Laser Capture Microdissection of Mammalian Tissue
16:34

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

17.5K

Area of Science:

  • Genomics
  • Molecular Biology
  • Spatial Transcriptomics

Background:

  • Understanding gene expression within its spatial context is crucial for biological research.
  • Existing methods may lack the resolution or throughput to comprehensively analyze spatial gene expression patterns.

Purpose of the Study:

  • To introduce and validate Geographical position sequencing (Geo-seq) as a method for spatial gene expression analysis.
  • To demonstrate the capability of Geo-seq for high-throughput transcriptomic profiling at specific spatial coordinates within tissues.

Main Methods:

  • Geo-seq combines laser capture microdissection with transcriptome sequencing.
  • It involves region-specific microdissection of tissue sections.
  • Subsequent RNA extraction, cDNA preparation, and high-throughput sequencing are performed.

Main Results:

  • Geo-seq enables comprehensive gene expression analysis within the spatial context.
  • The method is robust across various tissue types for in-depth gene expression studies.
  • It allows for the determination of transcript abundance and overall transcriptomic architecture.
  • High-throughput profiling of over 8000 genes from a single sampled spot is achievable.

Conclusions:

  • Geo-seq provides a powerful tool for dissecting spatial gene expression.
  • This technique facilitates a deeper understanding of tissue architecture and gene function in situ.
  • Geo-seq offers a robust and high-throughput approach for spatial transcriptomic studies.