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Updated: Feb 5, 2026

Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
Published on: May 4, 2011
Self-powered cathodic detection of dissolved oxygen using a paper-based biofuel cell
Isao Shitanda1,2, Riko Ohkura1, Noya Loew1
1Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science Noda 2641 Yamazaki Chiba 278-8510 Japan shitanda@rs.tus.ac.jp.
Abstract:
Herein we report the self-powered biosensor for detection of dissolved oxygen (DO) detection using a paper-based enzymatic biofuel cell (BFC) employing screen-printed electrodes composed of MgO-templated mesoporous carbon (MgOC). The sensor used an anode modified by flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) and a cathode modified by bilirubin oxidase (BOD) to enable selective oxygen reduction under glucose-rich conditions. Electrochemical analyses revealed a linear relationship between the cathodic current and DO concentration over the range of 0-22 mg L-1, with a maximum power output of 398 µW cm-2 at 20 mg L-1 DO. The biosensor system was successfully used to quantify DO in both pure water and a commercial soft drink, without requiring external power sources. These findings demonstrate the feasibility of low-cost, disposable, and scalable DO sensing by using cathode-targeting enzymatic BFCs, thereby opening new avenues for environmental and food quality monitoring.
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