Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Classification of Skeletal Muscle Fibers01:48

Classification of Skeletal Muscle Fibers

59.5K
Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
59.5K
Overview of Skeletal Muscle01:15

Overview of Skeletal Muscle

14.7K
Skeletal muscles are composed of a bundle of muscle fibers and are attached to bones through tendons. Each skeletal muscle fiber is a single muscle cell. The sarcolemma, the plasma membrane of a skeletal muscle cell, consists of a lipid bilayer and glycocalyx that supports muscle fibers. The sarcolemma extends into the muscle cells to form tubular structures called transverse or T-tubules. Each side of the T-tubules consists of a membrane-bound structure called the sarcoplasmic reticulum,...
14.7K
Relaxation of Skeletal Muscles01:29

Relaxation of Skeletal Muscles

5.9K
The period of muscle contraction primarily influences the duration of stimulation at the neuromuscular junction (NMJ), the presence of free calcium ions in the sarcoplasm, and the availability of energy or ATP to support contractions.
When an action potential reaches the axon terminal, it depolarizes the membrane and opens voltage-gated sodium channels. Sodium ions enter the cell, further depolarizing the presynaptic membrane. This depolarization causes voltage-gated calcium channels to open....
5.9K
Disorders of the Skeletal Muscle01:28

Disorders of the Skeletal Muscle

1.9K
The clinical conditions affecting the skeletal muscle tissue are broadly categorized as musculoskeletal and neuromuscular disorders.
Musculoskeletal disorders
Musculoskeletal disorders involve injuries and conditions affecting the skeletal muscles and associated connective tissues. These disorders can arise from acute biomechanical stresses or chronic overuse and can occur across different age groups. Common injuries include sprains, fractures, and muscular strains, often resulting from...
1.9K
Naming Skeletal Muscles01:19

Naming Skeletal Muscles

4.1K
The naming of the approximately 700 muscles in the human body is based on a set of criteria designed to provide descriptive information about each muscle, making it easier to identify and remember them.
The key factors used in naming muscles include:
4.1K
Skeletal Muscle Anatomy00:55

Skeletal Muscle Anatomy

92.8K
Skeletal muscle is the most abundant type of muscle in the body. Tendons are the connective tissue that attaches skeletal muscle to bones. Skeletal muscles pull on tendons, which in turn pull on bones to carry out voluntary movements.
92.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

UBA1 knockdown dysregulates the levels of UBA1-sensitive proteins and impairs muscle function in Drosophila and mice.

Disease models & mechanisms·2026
Same author

Mitochondrial integrated stress response activation creates a therapeutic vulnerability to MCL-1 inhibition in acute myeloid leukemia.

Cell death & disease·2026
Same author

A mutation in the nuclear speckle and splicing factor SRRM2 is associated with multisystem proteinopathy and causes dysregulation of synapse-associated genes.

RNA (New York, N.Y.)·2026
Same author

Ground Truth-Based Evaluation of False Discovery Rate and Statistical Power in DIA Proteomics.

bioRxiv : the preprint server for biology·2026
Same author

The mannosyltransferase DPM1 regulates the activity of ER-stress sensor IRE1 in colorectal cancer.

Nature communications·2026
Same author

JUMPlion improves quantitative DIA proteomics through ion-level recovery of missing values.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Feb 6, 2026

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
09:29

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

8.9K

Largely Distinct Post-Translational Modifications Differentiate Skeletal Muscle Wasting Caused by Cancer,

Anna Stephan1, Flavia A Graca1, Suresh Poudel2

  • 1Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

Journal of Cachexia, Sarcopenia and Muscle
|February 4, 2026
PubMed
Summary

Skeletal muscle wasting is linked to distinct post-translational modifications (PTMs). A specific PTM, Lrpprc P27 dihydroxylation, declines across various conditions, contributing to muscle weakness.

Keywords:
Leigh syndromeLrpprcagingatrophycancer cachexiapost‐translational modificationssarcopeniaskeletal muscle weakness

More Related Videos

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications
10:12

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

3.6K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.3K

Related Experiment Videos

Last Updated: Feb 6, 2026

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
09:29

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

8.9K
Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications
10:12

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

3.6K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.3K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Muscle Physiology

Background:

  • Skeletal muscle wasting and weakness are key features of various diseases.
  • Previously, muscle wasting was thought to stem from common transcriptional changes.
  • Recent findings indicate that distinct stimuli induce muscle wasting through largely different mRNA and protein alterations.

Purpose of the Study:

  • To investigate post-translational modifications (PTMs) associated with muscle wasting.
  • To identify common and stimulus-specific PTMs in muscle atrophy.
  • To elucidate the role of Lrpprc P27 dihydroxylation in muscle weakness.

Main Methods:

  • Utilized the JUMPptm pipeline for mass spectrometry analysis of modified peptides.
  • Examined PTMs in mouse models of muscle wasting induced by cancer, dexamethasone, and aging.
  • Performed electroporation experiments with wild-type and mutant Lrpprc to assess functional impact.

Main Results:

  • Most regulated PTMs were stimulus-specific, with only a few shared across conditions.
  • P27 dihydroxylation of Lrpprc (leucine-rich pentatricopeptide repeat containing) consistently declined with muscle wasting.
  • Electroporation of a dihydroxylation-resistant Lrpprc variant reduced muscle force and impaired expression of muscle strength genes.

Conclusions:

  • Identified atrophy-associated PTMs as potential biomarkers for specific atrophic stimuli.
  • P27 dihydroxylation of Lrpprc serves as a general marker for muscle wasting.
  • The decline in Lrpprc P27 dihydroxylation contributes to muscle weakness in diverse catabolic conditions.