Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR01:32

PCR

238.1K
Overview
238.1K
Types of Errors: Detection and Minimization01:12

Types of Errors: Detection and Minimization

11.4K
Error is the deviation of the obtained result from the true, expected value or the estimated central value. Errors are expressed in absolute or relative terms.
Absolute error in a measurement is the numerical difference from the true or central value. Relative error is the ratio between absolute error and the true or central value, expressed as a percentage.
Errors can be classified by source, magnitude, and sign. There are three types of errors: systematic, random, and gross.
Systematic or...
11.4K
Sampling Methods: Sample Types01:18

Sampling Methods: Sample Types

3.3K
Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
3.3K
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

667
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
667
Titrimetric Methods: Types and Commonly Used Strategies01:08

Titrimetric Methods: Types and Commonly Used Strategies

2.5K
In chemistry, titrimetric methods are broadly classified into three types: volumetric, gravimetric, and coulometric. Volumetric titrations involve measuring the volume of a titrant of known concentration that is required to react completely with an analyte. In gravimetric titrations, the standard solution reacts with the analyte to form an insoluble precipitate, which is filtered, dried, and weighed. In coulometric titrations, current is applied to an electrochemical reaction until the reaction...
2.5K
Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches01:23

Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches

475
Biopharmaceutical studies constitute a vital field aiming to enhance drug delivery methods and refine therapeutic approaches, drawing upon diverse interdisciplinary knowledge. In research methodologies, the choice between controlled and non-controlled studies significantly influences the study's reliability and accuracy.
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
475

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phenolic acids in root exudates mediate laccase-induced formation of estrogen precipitation co-polymers.

Ecotoxicology and environmental safety·2026
Same author

Oxygen-driven shift from Pb(II) lattice incorporation to reversible surface complexation: Exploiting hydrothermal sludge DOM for high-purity vivianite recovery.

Water research·2026
Same author

The p. Arg<sup>1800</sup>His Mutation of Factor VIII Results in Moderate Hemophilia A Due to Defective Processing and Stability of Functional Protein.

Cell biology international·2026
Same author

Mechanistic role of lipid metabolism in foot-and-mouth disease virus (FMDV) replication.

Veterinary research·2026
Same author

Discovery of C2: A Novel Lead Compound for the Treatment of Gout and Hyperuricemia via Multi-Pathway Inhibition.

Archiv der Pharmazie·2026
Same author

Early reversal of cardiac remodeling in patients with new-onset persistent left bundle branch block after transcatheter aortic valve replacement.

Journal of cardiothoracic surgery·2026

Related Experiment Video

Updated: Feb 7, 2026

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

12.4K

Study on the detection method of Brucella differential type PCR.

Yajie Luo1, Yun Qin1, Hao Zhang1

  • 1College of Veterinary Medicine, Inner Mongolia Agricultural University, China.

Veterinary Immunology and Immunopathology
|February 5, 2026
PubMed
Summary

New PCR assays can distinguish between wild-type and vaccine strains of Brucella, improving diagnosis and control of zoonotic diseases in livestock. This aids veterinary quarantine and eradication efforts.

Keywords:
BrucellaBrucella S19 vaccineBrucellosis vaccine strain A19Multiplex PCR identificVeterinary diagnosis

More Related Videos

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
12:01

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

32.1K
Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues
07:17

Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues

Published on: August 23, 2024

1.8K

Related Experiment Videos

Last Updated: Feb 7, 2026

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

12.4K
A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
12:01

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

32.1K
Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues
07:17

Author Spotlight: Advancing the Detection of Low-Frequency Mutations in Cancer Tissues

Published on: August 23, 2024

1.8K

Area of Science:

  • Veterinary Microbiology
  • Molecular Diagnostics
  • Zoonotic Disease Control

Background:

  • Brucellosis control in developing countries relies on vaccination, quarantine, and culling.
  • Distinguishing natural Brucella infection from vaccine response is a diagnostic challenge.

Purpose of the Study:

  • Develop and optimize PCR assays to differentiate wild-type Brucella from vaccine strains.
  • Address the clinical diagnostic gap in veterinary medicine.

Main Methods:

  • Developed singleplex and multiplex PCR assays targeting six differential genetic loci.
  • Optimized primer concentrations and reaction conditions, including annealing temperature (60 °C).

Main Results:

  • Singleplex PCR showed high specificity for Brucella species and vaccine strains (A19, S19).
  • Hexaplex PCR accurately excluded non-target bacteria (e.g., Streptococcus, Salmonella).
  • Established detection sensitivities for S2, Rev.1, and S19 vaccine strains.

Conclusions:

  • The developed PCR methods enable simultaneous differentiation of wild-type and vaccine Brucella strains.
  • Provides a scientific basis for improved veterinary quarantine, disease eradication, and vaccine strategies.