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Related Experiment Video

Updated: Feb 7, 2026

Author Spotlight: Exploring Heat Shock Proteins in Malaria and Tuberculosis Infections

Author Spotlight: Exploring Heat Shock Proteins in Malaria and Tuberculosis Infections

Published on: March 8, 2024

Heat shock protein modulates cell expansion via ROS homeostasis.

Shulin Ren^1,2,3, Haiyan Wang^1,3, Yuling Jiao^4,5,6

¹College of Life Sciences, Capital Normal University, Beijing, 100048, China.

The New Phytologist

|February 6, 2026

Summary

Heat shock proteins (HSPs) regulate plant cell expansion. A specific HSP90 co-chaperone, PpNudC6, is crucial for directional cell growth in moss by controlling reactive oxygen species (ROS) and cell wall mechanics.

Keywords:

Physcomitrium patens NudC ROS Rboh cell wall mechanics heat shock protein homeostasis

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Area of Science:

Plant Biology
Molecular Cell Biology
Biochemistry

Background:

Heat shock proteins (HSPs) are vital for cellular processes but their roles in plant development are not fully understood.
Directional cell expansion is fundamental for plant growth and morphology, requiring precise regulation of cell wall properties.

Purpose of the Study:

To investigate the function of the HSP90 co-chaperone PpNudC6 in the moss Physcomitrium patens.
To elucidate the molecular mechanisms by which PpNudC6 influences cell expansion, cell wall integrity, and redox homeostasis.

Main Methods:

Generation and phenotypic characterization of ppnudc6 mutants in Physcomitrium patens.
Scanning electron microscopy (SEM) and atomic force microscopy (AFM) to analyze cell wall structure and mechanics.
Investigation of reactive oxygen species (ROS) homeostasis and NADPH oxidase activity.
Co-immunoprecipitation to identify interacting proteins.

Main Results:

ppnudc6 mutants exhibit shortened, thickened protonemal cells due to disrupted cellulose microfibril organization and cell wall stiffness gradients.
Loss of PpNudC6 function leads to abnormal ROS accumulation caused by ectopic activity of NADPH oxidase PpRbohD.
Inhibition of NADPH oxidases rescues the mutant phenotypes, confirming ROS overproduction as the cause of developmental defects.
PpNudC6 interacts with PpRACK1B and PpSGT1, forming a complex that likely modulates Rboh activity.

Conclusions:

PpNudC6 is essential for directional cell expansion in moss by mediating ROS production and maintaining cell wall mechanical anisotropy.
This study reveals a novel role for HSP complexes in regulating cell expansion through redox homeostasis and cell wall mechanics.
Findings provide insights into the interplay between chaperone function, redox balance, and cell wall dynamics during plant development.