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Published on: June 10, 2021
Point transformer for protein structural heterogeneity analysis using CryoEM
Muyuan Chen1, Muchen Li2,3, Renjie Liao2,3,4
1Division of CryoEM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Stanford University.
None:
Structural dynamics of macromolecules is critical to their structural-function relationship. Cryogenic electron microscopy (CryoEM) provides snapshots of vitrified protein at different compositional and conformational states, and the structural heterogeneity of proteins can be characterized through computational analysis of the images. For protein systems with multiple degrees of freedom, it is still challenging to disentangle and interpret the different modes of dynamics. Here, by implementing Point Transformer, a self-attention network designed for point cloud analysis, we are able to improve the performance of heterogeneity analysis on CryoEM data, and characterize the dynamics of highly complex protein systems in a more human-interpretable way.
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