Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Longitudinal Research02:20

Longitudinal Research

13.4K
Sometimes we want to see how people change over time, as in studies of human development and lifespan. When we test the same group of individuals repeatedly over an extended period of time, we are conducting longitudinal research. Longitudinal research is a research design in which data-gathering is administered repeatedly over an extended period of time. For example, we may survey a group of individuals about their dietary habits at age 20, retest them a decade later at age 30, and then again...
13.4K
Longitudinal Studies01:26

Longitudinal Studies

533
Longitudinal studies are also widely used in other medical and social science fields. For instance, in cardiovascular research, they can monitor patients' health over decades to identify risk factors for heart disease, such as high cholesterol or smoking, and evaluate the long-term effectiveness of preventive measures. Similarly, in mental health studies, researchers might follow individuals from adolescence into adulthood to understand the development and progression of conditions like...
533
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.7K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.7K
Microscopic Anatomy of Skeletal Muscles01:13

Microscopic Anatomy of Skeletal Muscles

23.2K
Skeletal muscle cells, also called muscle fibers, are distinctly elongated, multi-nucleated, slender biological units. They are packed with specialized structures designed to facilitate their primary function, which is contraction.
The muscle sarcolemma is a plasma membrane enclosing each muscle cell that conducts electrical signals called action potentials. The sarcolemma extends into the cell to form T-tubules, ensuring the neural impulses are uniformly distributed across the entire muscle...
23.2K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.9K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.9K
Woodward–Hoffmann Selection Rules and Microscopic Reversibility01:34

Woodward–Hoffmann Selection Rules and Microscopic Reversibility

4.0K
Electrocyclic reactions, cycloadditions, and sigmatropic rearrangements are concerted pericyclic reactions that proceed via a cyclic transition state. These reactions are stereospecific and regioselective. The stereochemistry of the products depends on the symmetry characteristics of the interacting orbitals and the reaction conditions. Accordingly, pericyclic reactions are classified as either symmetry-allowed or symmetry-forbidden. Woodward and Hoffmann presented the selection criteria for...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Servo-Actuated 3D-Printed Disposable Microvalves for Automated, Scalable Organoid Culture in Standard Incubators.

bioRxiv : the preprint server for biology·2026
Same author

Cloud-connected pluripotent stem cell platform enhances scientific identity in underrepresented students.

Stem cell reports·2026
Same author

Cerebrovascular vulnerability and fibrosis in human brain aneurysms.

Nature neuroscience·2026
Same author

"Better Than Nothing" or Not Enough? User-Centered Reflections on AI-Generated Audio Descriptions Across Media Formats.

ASSETS. Annual ACM Conference on Assistive Technologies·2026
Same author

Spatially defined axonal guidance in neural organoids with micropatterned microfluidic channels.

bioRxiv : the preprint server for biology·2026
Same author

SpikeLab: Agentic tools for spike data analysis.

bioRxiv : the preprint server for biology·2026
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Feb 7, 2026

Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
10:30

Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

Published on: September 2, 2015

10.7K

A Modular In-Incubator Microscope for Longitudinal Live Cell Microscopy.

Drew Ehrlich, Yohei Rosen, Santhosh Arul

    Biorxiv : the Preprint Server for Biology
    |February 6, 2026
    PubMed
    Summary
    This summary is machine-generated.

    We developed an automated in-incubator microscope for long-term live cell imaging. This system overcomes limitations of manual methods, enabling high-frequency, extended duration studies of dynamic biological processes.

    More Related Videos

    Author Spotlight: Mitochondrial Remodeling in Skeletal Muscle
    10:53

    Author Spotlight: Mitochondrial Remodeling in Skeletal Muscle

    Published on: December 1, 2023

    4.4K
    Monitoring Neuronal Survival via Longitudinal Fluorescence Microscopy
    07:02

    Monitoring Neuronal Survival via Longitudinal Fluorescence Microscopy

    Published on: January 19, 2019

    6.9K

    Related Experiment Videos

    Last Updated: Feb 7, 2026

    Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
    10:30

    Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

    Published on: September 2, 2015

    10.7K
    Author Spotlight: Mitochondrial Remodeling in Skeletal Muscle
    10:53

    Author Spotlight: Mitochondrial Remodeling in Skeletal Muscle

    Published on: December 1, 2023

    4.4K
    Monitoring Neuronal Survival via Longitudinal Fluorescence Microscopy
    07:02

    Monitoring Neuronal Survival via Longitudinal Fluorescence Microscopy

    Published on: January 19, 2019

    6.9K

    Area of Science:

    • Cell Biology
    • Microscopy
    • Bioengineering

    Background:

    • Longitudinal live cell imaging is crucial for understanding dynamic cellular changes.
    • Manual microscopy is labor-intensive, limits imaging frequency, and disrupts cellular environments, hindering scalability and data quality.
    • Existing automated systems often face constraints related to cost, size, and inflexibility.

    Purpose of the Study:

    • To present an automated, in-incubator epifluorescence microscope for long-term live cell imaging.
    • To overcome the limitations of conventional and existing automated imaging platforms.
    • To enable high-frequency, extended duration imaging for detailed analysis of biological processes.

    Main Methods:

    • Designed a modular, automated in-incubator epifluorescence microscope.
    • Integrated features such as multi-fluorescence imaging, automated plate scanning, and configurable light sources.
    • Positioned external electronics outside the incubator to enhance thermal stability and reliability.

    Main Results:

    • The system supports long-term, continuous, high-frequency live cell imaging.
    • It offers compatibility with multiple plate formats and fluidic automation devices.
    • Improved thermal stability and operational reliability were achieved by externalizing control components.

    Conclusions:

    • The developed automated microscope enables scalable, high-resolution, long-term live cell imaging.
    • This platform provides rich data for quantifying time-dependent tissue phenotypes and transient biological processes.
    • It represents a significant advancement for dynamic biological system characterization.