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Updated: Feb 8, 2026

Glycan Profiling of Plant Cell Wall Polymers using Microarrays
Published on: December 17, 2012
Protocol for simultaneous profiling of N- and O-glycans on glycoproteins using a one-pot format
Kevan T Knizner1, Darren T Gass1, Yasuyuki Matsumoto1
1United States Food and Drug Administration, Center for Drug Evaluation and Research, Office of Pharmaceutical Quality, Silver Spring MD 20993, USA.
None:
Here, we present a protocol to simultaneously release and profile N- and O-glycans from glycoproteins in a one-pot format. We describe steps for protein reduction, alkylation, N-glycan release, Proteinase K digestion, HILIC enrichment, N-glycan reduction, N-/O-glycan permethylation while releasing O-glycans, and analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). For complete details on the use and execution of this protocol, please refer to Ortega-Rodriguez et al.1.
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