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Microwave time tunes broccoli-stem pectin fine structure and calcium gelation for sustained resveratrol release
Xinyuan Niu1, Dan Li2, Huiyao Zhang1
1College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271000, PR China.
Abstract:
In this study, the effects of microwave-assisted (MAE) extraction on the structural, functional and gel properties of broccoli stem pectin (BSP) were investigated. BSP was predominantly homogalacturonan (HG)-type pectin. BSP-150 s exhibited the highest galacturonic acid (GalA, 66.9 ± 0.5%), the largest molecular weight (Mw, 216.0 ± 0.7 kDa), and a moderate degree of esterification (DE, 34.6 ± 0.6%), yielding Ca2+-cross-linked hydrogels with the greatest hardness and water-holding capacity. Thermogravimetry analysis indicated higher onset and peak decomposition temperatures at 150 s, while XRD diffraction analysis revealed slightly denser amorphous packing. Multivariate analysis (PCA/PLSR) further demonstrated that GalA and Mw were the principal positive predictors of hardness and WHC, with ζ-potential contributing additively. Moreover, BSP-150 hydrogels exhibited effective resveratrol (RES) encapsulation and controlled release. These findings confirm that microwave-assisted (MAE) is an efficient method for obtaining broccoli stem pectin with superior gelation capacity and potential applications.
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