Related Experiment Video
Updated: Feb 9, 2026

A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana
Published on: November 9, 2013
BoMYB96 and BoMYB2 positively regulate seed germination by inhibiting the transcription of BoABI5
Zhen Shen1, Shuhua Huang2, Qingqing Pang2
1Hybrid Rapeseed Research Center of Shaanxi Province, Yangling, Shaanxi, 712100, China; College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Abstract:
The bZIP transcription factor ABI5 (Abscisic acid insensitive 5) plays a central role in regulating responses to ABA (Abscisic acid) signals during seed germination and early growth. In Arabidopsis thaliana, ABI5 is strongly induced by ABA, and high ABI5 expression inhibits seed germination. Plant MYB (Myeloblastosis) proteins respond to multiple hormonal signals, including ABA. Moreover, many R2R3 MYB transcription factors show functional similarities to ABI5; however, regulation of ABI5 transcriptional activity by R2R3 MYB factors during seed germination remains insufficiently characterized. In this study, we found that BoMYB96 and BoMYB2 bind the BoABI5 promoter and inhibit its transcription. A. thaliana and Brassica napus lines overexpressing BoMYB96 or BoMYB2 exhibited ABA insensitivity when exposed to exogenous ABA, which increased rapeseed germination rates under ABA treatment. These results demonstrate functional conservation of AtMYB96 and AtMYB2 in ABA signaling and expand understanding of ABI5 transcriptional regulation in the genomes of Brassica oleracea and B. napus.
More Related Videos
07:03Reliable Method for Assessing Seed Germination, Dormancy, and Mortality under Field Conditions
Published on: November 6, 2016
07:19Asymbiotic Germination and Leaf Explant-Based Regeneration of the Endangered Medicinal Orchid Hemipilia cucullata from Mature Seeds
Published on: September 19, 2025
Related Concept Videos
Master Transcription Regulators
Master Transcription Regulators
Cooperative Binding of Transcription Regulators
Cooperative Binding of Transcription Regulators
Positive Regulator Molecules
Positive Regulator Molecules