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Modification of structural and functional properties of camelina protein treated with ultrasound-assisted enzymatic
Ting Li1, Chuping Zhang1, Jianfen Ye2
1State Key Laboratory of Food Science and Resources, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
Abstract:
This study is aimed to improve the functional properties by subjecting camelina protein (CP) for ultrasound treatment (360 W, 15 min), followed by enzymatic hydrolysis (0-120 min). The solubility of CP was continuously improved after ultrasound-assisted enzymatic treatment (UAET). UAET decreased the molecular weight of protein as revealed by SDS-PAGE result. A significant increase of random coil (from 27.16% to 37.81%) was observed after treatment, indicating the enhanced structural flexibility. Morphological analysis manifested that a network-like structure formed through the interaction between released peptides, which was conductive to improve the stability of interfacial film. Accordingly, with hydrolysis time of 120 min, the foaming capacity and emulsifying capacity were increased by 22% and 9%, respectively, which were related to the improved solubility and structural changes. The improvement in antioxidant capacity after treatment was resulted from the release of peptides with low molecular weight that can interact with radicals.
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