Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Predicting Molecular Geometry02:27

Predicting Molecular Geometry

46.0K
VSEPR Theory for Determination of Electron Pair Geometries
46.0K
Prediction Intervals01:03

Prediction Intervals

3.4K
The interval estimate of any variable is known as the prediction interval. It helps decide if a point estimate is dependable.
However, the point estimate is most likely not the exact value of the population parameter, but close to it. After calculating point estimates, we construct interval estimates, called confidence intervals or prediction intervals. This prediction interval comprises a range of values unlike the point estimate and is a better predictor of the observed sample value, y. 
3.4K
End Point Prediction: Gran Plot01:07

End Point Prediction: Gran Plot

1.2K
A Gran plot is used to predict the equivalence volume or endpoint of a potentiometric or acid-base titration without reaching the endpoint. Typically, titration data is collected as a function of the titrant's volume up to a point less than the equivalence volume and then transformed into a linear format. The straight line is extended to the x-axis, indicating the necessary titrant volume to achieve the equivalence point.
For potentiometric titration, the Gran plot is created by plotting...
1.2K
Sensitivity, Specificity, and Predicted Value01:13

Sensitivity, Specificity, and Predicted Value

1.4K
In healthcare diagnostics, laboratory tests play a crucial role in identifying and diagnosing a wide range of medical conditions. However, interpreting test results is not always straightforward. An abnormal test result does not always confirm the presence of a disease, just as a normal result does not guarantee its absence. To assess the reliability of these diagnostic tools, healthcare practitioners rely on two key statistical indicators: sensitivity and specificity.
Sensitivity is the...
1.4K
Predicting Reaction Outcomes02:24

Predicting Reaction Outcomes

10.9K
Kinetics describes the rate and path by which a reaction occurs. In contrast, thermodynamics deals with state functions and describes the properties, behavior, and components of a system. It is not concerned with the path taken by the process and cannot address the rate at which a reaction occurs. Although it does provide information about what can happen during a reaction process, it does not describe the detailed steps of what appears on an atomic or a molecular level. On the other hand,...
10.9K
Predicting Products: Substitution vs. Elimination02:52

Predicting Products: Substitution vs. Elimination

14.8K
When a nucleophile and an alkyl halide react, nucleophilic substitution and β-elimination reactions compete to generate products.
The following factors can influence the mechanisms competing against each other:
14.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improving Generalizability in Whole-Cell Antibiotic Discovery Through Active Learning.

bioRxiv : the preprint server for biology·2026
Same author

The protein binding domains of staphylococcal protein A fold independently and form an N- to C-terminal gradient of increasing stability.

bioRxiv : the preprint server for biology·2026
Same author

A Consensus Approach to the Incorporation of Total Neoadjuvant Therapy in a Treatment Algorithm for Stage I-III Resectable Rectal Cancer.

Current oncology (Toronto, Ont.)·2026
Same author

A deformylase inhibitor expands therapeutic options for Lyme disease.

Research square·2026
Same author

Nursing Home Entry in Sweden: The Role of Sociodemographic Factors, Health, and Dementia.

Journal of the American Medical Directors Association·2026
Same author

Kinetics of Sevoflurane Vaporized Directly in Extracorporeal Membrane Oxygenators-Ex Vivo Findings and Pharmacokinetic/Pharmacodynamic Simulations.

European journal of drug metabolism and pharmacokinetics·2026

Related Experiment Video

Updated: Feb 9, 2026

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
09:42

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

Published on: June 19, 2012

12.8K

ABSEIL: A polypeptide helicity and ensemble prediction tool.

Hannah Hortman1, Ruiling A Zhang2, Roy G Hughes3

  • 1Trinity College, Duke University, Durham 27708, NC, USA.

Journal of Molecular Biology
|February 7, 2026
PubMed
Summary
This summary is machine-generated.

A new tool, ABSEIL, predicts protein helicity using helix-coil models and peptide circular dichroism data. It helps understand early protein folding events and intrinsically disordered regions.

Keywords:
Ensemble enumerationHelical peptide databaseHelicity predictionHelix-coil models

More Related Videos

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
16:02

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation

Published on: February 10, 2023

3.3K
Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes
12:24

Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

Published on: June 3, 2014

12.7K

Related Experiment Videos

Last Updated: Feb 9, 2026

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
09:42

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

Published on: June 19, 2012

12.8K
Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
16:02

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation

Published on: February 10, 2023

3.3K
Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes
12:24

Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

Published on: June 3, 2014

12.7K

Area of Science:

  • Protein folding and biophysics
  • Computational biology and bioinformatics

Background:

  • Nascent helicity in polypeptides is crucial for early protein folding events.
  • It influences the physical properties of intrinsically disordered protein regions.
  • Helix-coil models are statistical mechanical models describing coil-to-helix transitions.

Purpose of the Study:

  • To introduce A Bayesian Statistical Engine to Infer HeLicity (ABSEIL), a web tool for predicting protein secondary structure.
  • To enable users to predict overall and residue-specific helicity of polypeptide sequences.
  • To allow enumeration of helix-coil configurations and access to a database of peptide circular dichroism experiments.

Main Methods:

  • Empirical parameterization of helix-coil models using circular dichroism (CD) measurements.
  • Development of a Bayesian statistical engine (ABSEIL) for helicity prediction.
  • Web application architecture supporting anonymous usage and administrative management.

Main Results:

  • ABSEIL provides predictions for overall sequence helicity.
  • ABSEIL offers residue-specific helicity predictions.
  • ABSEIL enumerates helix-coil configurations by population.

Conclusions:

  • ABSEIL is a valuable tool for studying nascent helicity and protein folding.
  • The tool facilitates research on intrinsically disordered regions.
  • ABSEIL enhances the accessibility of predictive models and experimental data for the scientific community.