Exosomes from APCs ameliorate human skin fibroblast senescence via p53/p21 signaling pathway
Yong-Chao Zhang1, Mei-Shui Wang2, Biao Wang2
1Research Institute of Plastic and Aesthetic Surgery, the First Affiliated Hospital, Fujian Medical University, China; Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, China.
Abstract:
Exosomes have emerged as important resources in skin regenerative medicine. However, only a limited number of studies have demonstrated the anti-aging effects of progenitor cell-derived exosomes. In addition, the development of novel effective progenitor cell-based therapies is crucial for the treatment of skin aging. In this study, the viability and proliferation of human adipose-derived progenitor cells (APCs) from young (18-25 years) and old (60-67 years) donors were compared. Exosomes derived from young (yAPC-Exos) and old (oAPC-Exos) APCs were collected and characterized, and their effects on senescent human dermal fibroblasts (HDFs), as well as the underlying molecular mechanisms, were investigated. The proliferation capacity of aged APCs was significantly reduced. Both yAPC-Exos and oAPC-Exos promoted HUVEC migration and tube formation, as well as HDF migration. Exosome treatment decreased intracellular reactive oxygen species levels and alleviated aging-associated phenotypes in senescent HDFs. These effects occurred primarily through p21 and p53 downregulation and SIRT1 upregulation. Notably, yAPC-Exos exerted more pronounced anti-senescent effects than oAPC-Exos. Taken together, yAPC-Exos may represent an effective therapeutic strategy for aging-related skin pathologies and cosmetic applications.
Related Concept Videos
Notch Signaling Pathway
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
Hedgehog Signaling Pathway
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Non-Canonical Wnt Signaling Pathways
Canonical Wnt Signaling Pathway
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The...


