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Updated: Feb 10, 2026

A High-throughput Shigella-specific Bactericidal Assay
Published on: February 27, 2019
Bactericidal Activities of Copolymers Incorporating Formylphenyl Motif for Targeting Surface Proteins
Esteban Bautista1, Melody Sun1, Michael Colwin2,3
1Department of Chemistry, University of California Irvine, Irvine, California 92697, United States.
Abstract:
We developed a set of intrinsic antimicrobial copolymers equipped with a dual-binding mechanism for the bacterial cell surface, targeting both the anionic bacterial membrane through electrostatic interactions and surface proteins through reversible imine formation. These copolymers, containing formylphenyl and quaternary ammonium functional groups, were systematically evaluated for their biological activity as the chemical composition and architecture were varied. These bactericidal polymers exhibit potent antimicrobial activity in NB media against both Staphylococcus epidermidis and Escherichia coli, while also demonstrating excellent in vitro biocompatibility against mammalian and red blood cells. This work expands the chemical repertoire of intrinsic antimicrobial polymers that coalesce with bacterial matter.
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