Intra-and Inter-Analyzer Imprecision of Cell Population Data on Sysmex XN-10

Area of Science:

• Hematology
• Clinical Diagnostics
• Medical Technology

Background:

• Cell population data (CPD) offer advanced insights into leukocytes beyond traditional counts.
• Clinical adoption of CPD requires proven analytical precision and inter-instrument consistency.

Purpose of the Study:

• To evaluate the analytical precision and reproducibility of CPD parameters on the Sysmex XN-10 hematology analyzer.
• To assess the suitability of specific CPD parameters for clinical use, especially in infection and sepsis diagnostics.

Main Methods:

• Two K2EDTA blood samples were analyzed in ten replicates on two Sysmex XN-10 analyzers.
• Intra- and inter-analyzer imprecision were calculated using coefficients of variation (CV%).

Main Results:

• Intra-analyzer CV% ranged from 0.2-7.9% and inter-analyzer CV% from 0.6-9.8%.
• Neutrophil, lymphocyte, and monocyte CPD parameters showed low intra-/inter-analyzer CVs (0.2-7.9%/0.6-9.8%).
• NE-SFL and MO-X parameters exhibited very low imprecision (intra-analyzer CV% 0.7-0.9% and 0.2-0.5%; inter-analyzer CV% 0.9-1.7%).

Conclusions:

• The Sysmex XN-10 demonstrates excellent reproducibility for CPD parameters.
• Low variability in NE-SFL and MO-X supports their reliability as clinical parameters for infection and sepsis diagnostics.