Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hydrogen Bonds00:26

Hydrogen Bonds

134.2K
Hydrogen bonds are weak attractions between atoms that have formed other chemical bonds. One of these atoms is electronegative, like oxygen, and has a partial negative charge. The other is a hydrogen atom that has bonded with another electronegative atom and has a partial positive charge.
Hydrogen Bonds Control the World!
Because hydrogen has very weak electronegativity when it binds with a strongly electronegative atom, such as oxygen or nitrogen, electrons in the bond are unequally shared....
134.2K
Hydrogen Bonds01:04

Hydrogen Bonds

14.9K
A hydrogen bond is formed when a weakly positive hydrogen atom already bonded to one electronegative atom (for example, the oxygen in the water molecule) is attracted to another electronegative atom from another polar molecule, such as water (H2O), hydrogen fluoride (HF), or ammonia (NH3). The huge electronegativity difference between the H atom (2.1) and the atom to which it is bonded (4.0 for an F atom, 3.5 for an O atom, or 3.0 for an N atom), combined with the very small size of an H atom...
14.9K
What are Lipids?01:38

What are Lipids?

220.9K
Overview
220.9K
What are Lipids?01:31

What are Lipids?

11.4K
Lipids function as structural components of cellular membranes, in addition to acting as energy reservoirs and signaling molecules. They are thus crucial to all living organisms.  The three biologically important classes of lipids are triglycerides, phospholipids, and steroids.
Non-Polar and Hydrophobic Characteristics of Lipids
Lipids are a structurally and functionally diverse group of hydrocarbons—compounds consisting of carbon and hydrogen atoms. The carbon-carbon and...
11.4K
Structure of Lipids03:38

Structure of Lipids

99.1K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
99.1K
Radical Formation: Abstraction00:47

Radical Formation: Abstraction

4.4K
The electron of an atom can be abstracted from a compound by a relatively unstable radical to generate a new radical of relatively greater stability. For example, an initiator which forms radicals by homolysis can abstract a suitable species like a hydrogen atom or a halogen atom from a compound to generate a new radical. This ability of radicals to propagate by abstraction is a crucial feature of radical chain reactions.
Even though homolysis produces radicals, it is different from radical...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enzyme-Activatable Fluorogenic Probes: Design Strategies, Biomedical Applications, and Future Perspectives.

Journal of the American Chemical Society·2026
Same author

Study motivations and perceived academic and career returns among international students in clinical medicine education in China: an interview study.

BMC medical education·2026
Same author

Acidification Dominates over Hypoxia in Controlling Estuarine Nitrogen Removal Dynamics under Coupled Stressors.

Environmental science & technology·2026
Same author

Single-precursor-derived carbon and nitrogen co-doped CuO nanozymes for colorimetric detection of tetracycline.

Analytical methods : advancing methods and applications·2026
Same author

A Rationally Engineered MAGL-Activatable Fluorogenic Probe Enables Efficient Discovery of Anti-Inflammatory and Hepatoprotective Agents.

Journal of medicinal chemistry·2026
Same author

Utility of the Iwate Difficulty Scoring System for the Stratification of Laparoscopic Right Hepatectomies: An International Multicenter Study.

Journal of hepato-biliary-pancreatic sciences·2026

Related Experiment Video

Updated: Feb 10, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
03:55

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

4.2K

Early Ferroptosis Detection Targeting Lipid Hydrogen Abstraction.

Cankun Li1, Cheng Yao1, Ruiqi Su2,3

  • 1State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China.

Journal of the American Chemical Society
|February 9, 2026
PubMed
Summary

Researchers developed novel fluorogenic probes (LHA585, LHA675, LHA930) for early ferroptosis detection by targeting lipid hydrogen abstraction. These probes offer superior sensitivity and speed compared to existing methods.

More Related Videos

Solid Lipid Nanoparticles SLNs for Intracellular Targeting Applications
08:19

Solid Lipid Nanoparticles SLNs for Intracellular Targeting Applications

Published on: November 17, 2015

18.7K
Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection
11:56

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Published on: October 25, 2013

14.7K

Related Experiment Videos

Last Updated: Feb 10, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
03:55

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

4.2K
Solid Lipid Nanoparticles SLNs for Intracellular Targeting Applications
08:19

Solid Lipid Nanoparticles SLNs for Intracellular Targeting Applications

Published on: November 17, 2015

18.7K
Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection
11:56

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

Published on: October 25, 2013

14.7K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Chemical Biology

Background:

  • Ferroptosis is a regulated cell death pathway driven by iron-dependent lipid peroxidation.
  • Early detection of ferroptosis is crucial for effective diagnosis and treatment.
  • Lipid hydrogen abstraction initiates the ferroptosis cascade, making it a key target for early detection.

Purpose of the Study:

  • To develop novel fluorogenic probes for the specific detection of lipid hydrogen abstraction.
  • To enable earlier and more sensitive detection of ferroptosis.
  • To facilitate mechanistic studies of ferroptosis.

Main Methods:

  • Design and synthesis of three fluorogenic probes: LHA585 (red), LHA675 (deep-red), and LHA930 (near-infrared).
  • Utilized a 4-phenyl-3-methylbut-2-enyl moiety as a lipid mimic to detect oxidative radicals.
  • Evaluated probe performance in vitro (OGD/R model) and in vivo.

Main Results:

  • The LHA probes specifically detect lipid hydrogen abstraction, the initiating event in ferroptosis.
  • LHA585 demonstrated an ~8-hour earlier fluorescence turn-on than the gold standard in an in vitro model.
  • LHA930 proved effective for in vivo ferroptosis detection.

Conclusions:

  • The LHA probes represent a significant advancement in ferroptosis sensing technology.
  • These probes enable earlier and more precise detection of ferroptosis.
  • The LHA probes are valuable tools for investigating lipid-peroxidation-driven cell death.