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A novel approach for discriminating lipophilic bisphosphonate-based pharmaceuticals using a potentiometric array
Tatiana V Shishkanova1, Annemarie Skálová-Coufal1, Jaroslav Otta2
1Department of Analytical Chemistry, University of Chemistry and Technology, Prague, Technická 5, Prague 6, 166 28, Czech Republic. tatiana.shishkanova@vscht.cz.
Abstract:
Bisphosphonates are pharmaceutical compounds commonly used in the treatment of osteoporosis, Paget's disease, and multiple myeloma. Their lipophilicity was evaluated using a sensor array composed of poly(vinyl chloride) (PVC)-based ion-selective membranes (ISMs), modified with a polyaniline (PANI) layer that affects surface properties and acts as an anion-exchanger. The lipophilicity of bisphosphonates including ibandronate, clodronate, risedronate, and alendronate was analyzed in both standard and commercial samples. The influence of membrane composition (presence or absence of an anion-exchanger) and PANI deposition conditions (monomer form and/or salt content) on membrane surface properties (lipophilicity and signal stability) was investigated and confirmed using surface-wetting characterization, SEM-EDS mapping and potentiometry. Principal component analysis (PCA) enabled discrimination among the bisphosphonates based on their lipophilicity and revealed distinct contributions of individual ISMs in both standard and commercial samples.
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