Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

14.6K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
14.6K
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

668
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
668
Three Developmental Domains01:29

Three Developmental Domains

1.2K
Human development is typically examined across three main domains: physical, cognitive, and socio-emotional. These domains represent the significant areas of change and continuity throughout the lifespan, from infancy to late adulthood.
Physical Development
Physical processes, also known as maturation, encompass the biological changes that occur across an individual's life. These changes begin with genetic inheritance and continue through various stages, including growth in height and weight,...
1.2K
Membrane Domains01:18

Membrane Domains

7.2K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
7.2K
Three-Domain System of Life01:21

Three-Domain System of Life

1.3K
Ribosomal RNA (rRNA) sequence analysis revealed three distinct groups of cells: eukaryotes, bacteria, and archaea. In 1978, Carl R. Woese proposed the concept of domains, a taxonomic level above kingdoms, to differentiate these groups. He suggested that archaea and bacteria, despite their similar appearance, represent separate domains. Domains differ in rRNA, membrane lipid structure, transfer RNA, and antibiotic sensitivity.In this classification, animals, plants, and fungi belong to the...
1.3K
Electrophoresis: Overview01:20

Electrophoresis: Overview

4.1K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electrokinetic separation of bacteriophage ϕKZ from bacterial cells.

The Analyst·2026
Same author

Nonantiperiodic Nonlinear Electrophoresis of Colloidal Particles.

Analytical chemistry·2025
Same author

Fifty Years of Nonlinear Electrophoresis.

Electrophoresis·2025
Same author

Predicting the retention time of microparticles in electrokinetic migration.

The Analyst·2025
Same author

Tuning the Migration Order in Electrokinetic Separations of <i>Saccharomyces cerevisiae</i> Cells.

Analytical chemistry·2025
Same author

Short Communication: Ultralow Voltage Electrokinetic Particle Trapping in DC-iEK Devices Using 9 V Alkaline Batteries as Power Supply.

Electrophoresis·2024

Related Experiment Video

Updated: Feb 11, 2026

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

29.4K

Electrophoresis: An Effective Viability Assessment Method across Cell Domains.

Jesus Espino1, Carlos A Mendiola-Escobedo1, Alaleh Vaghef-Koodehi1

  • 1Microscale Bioseparations Laboratory and Biomedical Engineering Department, Rochester Institute of Technology, 160 Lomb Memorial Drive, Rochester, New York 14623, United States.

Analytical Chemistry
|February 10, 2026
PubMed
Summary

This study introduces insulator-based electrokinetics (iEK) for rapid cell viability assessment. This novel method effectively separates viable from nonviable prokaryotic and eukaryotic cells using electrophoresis, offering broad applicability.

More Related Videos

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

47.3K
A Direct and Simple Method to Assess Drosophila melanogaster's Viability from Embryo to Adult
04:32

A Direct and Simple Method to Assess Drosophila melanogaster's Viability from Embryo to Adult

Published on: August 27, 2019

11.4K

Related Experiment Videos

Last Updated: Feb 11, 2026

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

29.4K
Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

47.3K
A Direct and Simple Method to Assess Drosophila melanogaster's Viability from Embryo to Adult
04:32

A Direct and Simple Method to Assess Drosophila melanogaster's Viability from Embryo to Adult

Published on: August 27, 2019

11.4K

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microfluidics

Background:

  • Cell viability assessment is crucial in research and drug development.
  • Existing electrokinetic methods like electrorotation and dielectrophoresis have limitations.
  • There is a need for robust, adaptable cell viability assessment techniques.

Purpose of the Study:

  • To explore insulator-based electrokinetic (iEK) systems for rapid cell viability assessment.
  • To demonstrate the broad efficacy of iEK across different cell types (prokaryotic and eukaryotic).
  • To utilize weak and moderate electric field regimes for adaptive cell separation.

Main Methods:

  • Utilized insulator-based electrokinetic (iEK) systems in a microfluidic setup.
  • Applied electrophoresis in weak and moderate electric field regimes.
  • Separated viable and nonviable cells based on differences in electromigration.

Main Results:

  • Successfully separated viable and nonviable cells from both bacterial (E. coli, S. Typhimurium) and yeast (S. cerevisiae) strains.
  • Achieved high resolution (Rs > 1.2) and good reproducibility across all tested conditions.
  • Demonstrated domain-agnostic discrimination, confirming broad efficacy.

Conclusions:

  • Insulator-based electrokinetic (iEK) electrophoresis is a novel and robust platform for cell viability assessment.
  • iEK systems offer rapid, quantitative, and high-resolution separation of viable and nonviable cells.
  • This technique has wide applicability across various scientific disciplines.