Reduction and Refinement of Genetically Altered Mouse Breeding: Controlling Quality and Quantity According to...

Area of Science:

• * Biomedical research
• * Animal models in science

Background:

• * Mice are the most common animal model globally.
• * In Europe, 50% of research mice are genetically modified, with 60% of transgenic mice unused.
• * Inefficient production leads to significant animal waste.

Purpose of the Study:

• * To present a rigorous framework for managing genetically modified mouse lines.
• * To optimize production strategies and minimize the generation of unused animals.
• * To promote responsible and efficient use of mouse models in research.

Main Methods:

• * Introduction of the DOPPUME framework: Define, Obtain, Protect, Produce, Use, Manage, Exchange.
• * Emphasis on clearly defining models, controlling genetic characteristics, and ensuring stability.
• * Tools for defining production goals and accurately estimating needs based on experimental plans.

Main Results:

• * The DOPPUME framework offers a structured approach to genetically modified mouse line management.
• * Proactive colony management and producing only necessary animals are key strategies.
• * The framework supports accurate production planning and resource allocation.

Conclusions:

• * Implementing the DOPPUME framework enhances the efficiency and responsibility of using mouse models.
• * This systematic approach aligns with and supports the 3Rs principles (Replace, Reduce, Refine).
• * Optimized production strategies are essential for sustainable and ethical scientific research.