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Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
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Expanding enzyme functionality through site-specific incorporation of noncanonical amino acids

Siddiq Pasha Shaik1, Anni Li2, Jiantao Guo1

  • 1Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA; The Nebraska Center for Integrated Biomolecular Communication (NCIBC), University of Nebraska-Lincoln, Lincoln, NE 68588, USA.

Trends in Biochemical Sciences
|February 10, 2026
PubMed

Keywords:
enzyme engineeringgenetic code expansionmetalloenzymesnoncanonical amino acidsorganocatalytic enzymesphotoenzymes

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