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Updated: Feb 12, 2026

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Computational Validation of Multi-Epitope mRNA Vaccine Targeting Streptococcus anginosus Surface Protein (TMPC) as an

Fei Zhu1,2,3, Yuying Luo1,2,3, Ziyou Zhou1,2,3

  • 1Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Microbiologyopen
|February 10, 2026
PubMed

Insights

A novel multi-epitope vaccine targeting Streptococcus anginosus, a bacterium linked to gastric cancer, was designed using Treponema pallidum membrane protein C (TMPC). Computational analysis predicts a strong immune response, paving the way for potential new treatments.

Area of Science:

  • Microbiology
  • Immunology
  • Vaccine Development

Background:

  • Streptococcus anginosus is associated with increased gastric cancer risk.
  • Current treatments lack vaccines and face antibiotic resistance challenges.

Purpose of the Study:

  • To design a multi-epitope vaccine targeting TMPC-positive streptococcal species, primarily S. anginosus.
  • To evaluate the vaccine construct's immunogenicity and stability through computational methods.

Main Methods:

  • Development of a TMPC-based multi-epitope vaccine construct.
  • Selection of B-cell and T-cell epitopes based on binding affinity, antigenicity, and immunogenicity.
  • Computational analysis including molecular docking and molecular dynamics simulations.
  • Modification into a messenger RNA (mRNA) vaccine for enhanced T-cell response.

Main Results:

  • The vaccine construct incorporates epitopes from nine TMPC-positive streptococcal species.
  • Immune simulations predicted robust humoral and cellular immune responses.
  • Molecular docking confirmed stable interactions with immune receptors (HLA and TLRs).

Conclusions:

  • A TMPC-based multi-epitope vaccine candidate was computationally designed against S. anginosus.
  • The vaccine shows potential for inducing significant immune responses.
  • Further animal studies are required to validate immunogenicity and protective efficacy.
Keywords:
Streptococcus anginosusgastric cancermRNA vaccinemolecular modelingmulti‐epitopes

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