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Intravaginal Progesterone Application as an Efficient and Reproducible Tool for Synchronizing the Mouse Estrous Cycle

Ban Sato1, Yutaka Yamashita1, Miyuki Shindo2

  • 1Laboratory of Regulatory Biology, Department of Life Sciences School of Agriculture, Meiji University Kawasaki Kanagawa Japan.

Reproductive Medicine and Biology
|February 11, 2026
PubMed
Summary

Intravaginal progesterone (P4) cream effectively synchronizes the mouse estrous cycle. This method improves reproductive efficiency and animal welfare by reducing discomfort and the need for vasectomized males.

Keywords:
animal welfareintravaginal applicationprogesteronepseudopregnant femalessynchronization of estrous cycle

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