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Influence on sCD40L Value According to ELISA Assay Kits
Background:
Recent studies show sCD40L as a potential biomarker for thrombotic risk and cancer progression. Accurate measurement of sCD40L level is critical for research and clinical applications. However, variations in preanalytic conditions, sample types and discrepancies in reference range across ELISA kits pose challenges to standardization in biobanking and research reproducibility.
Methods:
sCD40L levels were measured using two ELISA kits. Bayesian statistical methods defined reference ranges, and paired t-tests and Pearson's correlation assessed differences and correlation between kits.
Results:
The reference range for sCD40L using the R&D kit was 1,095.48 - 6,603.00 pg/mL, and for the Invitrogen kit, 1,620.00 - 10,405.00 pg/mL. There was a statistically significant difference between kits (p = 0.0019), and a strong correlation (r = 0.88) in serum samples.
Conclusions:
sCD40L reference values differ by ELISA kit, underscoring the need for institution-specific reference ranges. Serum, not plasma, is preferable for sCD40L measurement. Establishing standardized reference ranges will improve the reliability of sCD40L as a biomarker in research fields.
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