Evaluation of Rhesus and Cynomolgus Macaque Whole Blood During Prolonged Cold Storage

Area of Science:

• Veterinary Medicine
• Trauma Research
• Blood Storage

Background:

• Cold stored whole blood (WB) is increasingly used for massive hemorrhage resuscitation.
• Nonhuman primates (NHP) are crucial preclinical models for hemostatic research due to physiological similarity to humans.
• Current NHP trauma models often use autologous shed WB, not fully replicating human clinical scenarios.

Purpose of the Study:

• To evaluate the effects of prolonged 4°C storage on NHP WB.
• To characterize the storage lesion in rhesus and cynomolgus macaque WB.
• To assess the viability of using stored allogeneic WB in NHP resuscitation models.

Main Methods:

• WB was collected from rhesus and cynomolgus macaques and stored at 4°C in CPDA-1.
• Biochemical, hematologic, and hemostatic parameters were assayed daily for 4 days, then weekly up to 28 days.
• Data were analyzed using mixed effects analysis with multiple comparisons.

Main Results:

• Red blood cell counts, hemoglobin, and hematocrit remained stable through 28 days of storage.
• Lactate and potassium levels increased significantly, while sodium and pH decreased significantly.
• Hemostatic function significantly declined in both species from day 7 onwards.

Conclusions:

• A viable method for manufacturing stored NHP WB was established.
• The study characterized the storage lesion in NHP WB, with no overt interspecies differences observed.
• Utilizing stored allogeneic WB in future NHP resuscitation studies can enhance translational relevance.