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Updated: Feb 13, 2026

A Workflow for the Quantitative Assessment of the Endophytic and Epiphytic Bacterial Microbiomes of the Bark of Populus trichocarpa
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A Workflow for the Quantitative Assessment of the Endophytic and Epiphytic Bacterial Microbiomes of the Bark of Populus trichocarpa

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A discriminative primer design workflow enables selective metabarcoding, demonstrated using long-read sequencing of

Ting He1, Koert Jansonius1, Xiao Li1

  • 1Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands.

FEMS Microbiology Ecology
|February 11, 2026
PubMed

Keywords:
Amplicon sequencingOxford Nanopore Technologydiscriminative primer designhost-associated microbiomemetabarcoding, marker genes

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