Related Experiment Video

Updated: Feb 13, 2026

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
10:07

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

68.6K

Houpu Sanwu decoction alleviates rat intraperitoneal adhesion by regulating macrophage polarization and inhibiting

Mei Liu1, Quanxin Chen2, Yan Cui2

  • 1Department of Rehabilitation Medicine, Lanzhou University Second Hospital, Gansu 730000, China; The First Clinical Medical School, Gansu University of Chinese Medicine, Gansu 730000, China.

Tissue & Cell
|February 11, 2026
PubMed
Abstract

Insights

Houpu Sanwu decoction effectively reduces postsurgical intraperitoneal adhesion by modulating macrophage polarization and the SOCS/JAK2/STAT/PPAR-γ pathway.

Area of Science:

  • Surgical pathology
  • Immunology
  • Pharmacology

Background:

  • Intraperitoneal adhesion is a common complication after abdominal surgery, significantly impacting patient quality of life.
  • This study investigates the therapeutic potential of Houpu Sanwu decoction for treating intraperitoneal adhesion and elucidating its underlying molecular mechanisms.

Purpose of the Study:

  • To evaluate the efficacy of Houpu Sanwu decoction in reducing intraperitoneal adhesion in a rat model.
  • To explore the molecular mechanisms by which Houpu Sanwu decoction exerts its therapeutic effects, focusing on macrophage polarization and the SOCS/JAK2/STAT/PPAR-γ pathway.

Main Methods:

  • A rat model of abdominal adhesion was established, with animals randomized into sham, control, fluvastatin, and varying concentrations of Houpu Sanwu decoction groups.
  • Histopathological analysis (Hematoxylin and eosin, Masson staining) was performed on peritoneal tissues.
  • Protein levels of MCP-1 and serum levels of inflammatory cytokines (IL-1, IL-6, TNF-α, IL-4, IL-10) were quantified.
  • Gene and protein expression of the SOCS/JAK2/STAT/PPAR-γ pathway was assessed using RT-PCR and western blotting.

Main Results:

  • Houpu Sanwu decoction significantly decreased the incidence of intraperitoneal adhesion, with optimal effects observed at medium concentrations.
  • Treatment led to a reduction in MCP-1 protein expression and modulated macrophage polarization, decreasing M1 markers (IL-1, IL-6, TNF-α) and increasing M2 markers (IL-4, IL-10).
  • The decoction activated the SOCS1/STAT6/PPAR-γ pathway and inhibited the SOCS3/JAK2/STAT1 pathway, promoting M2 macrophage polarization.

Conclusions:

  • Houpu Sanwu decoction demonstrates significant therapeutic efficacy in alleviating intraperitoneal adhesion.
  • The mechanism involves the induction of M2-type macrophage polarization and modulation of the SOCS/JAK2/STAT/PPAR-γ signaling pathway.
Keywords:
Hopu Sanwu decoctionIntraperitoneal adhesionMacrophage polarizationSOCS/JAK2/STAT/PPAR-γ pathway

More Related Videos

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response12:50

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

7.0K
Author Spotlight: Exploring Huotan Jiedu Tongluo Decoction as an Antihypertensive Drug05:57

Author Spotlight: Exploring Huotan Jiedu Tongluo Decoction as an Antihypertensive Drug

Published on: May 17, 2024

1.2K

Related Concept Videos

Inflammatory Response01:28

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
17.0K
Inflammatory Response II: Inflammatory Exudate and Tissue Repair01:24

Inflammatory Response II: Inflammatory Exudate and Tissue Repair

The immune system's inflammatory response destroys the invading pathogen, permitting the tissue to heal. The changes during the cellular and vascular stages allow exudate formation at the site of inflammation. The inflammatory exudate released from the wound has high protein content and a specific gravity above 1.020.
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...
8.0K
Adhesion01:14

Adhesion

Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...
44.6K
Inflammatory Response I: Vascular and Cellular01:30

Inflammatory Response I: Vascular and Cellular

The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...
16.8K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
3.1K
Group Polarization01:01

Group Polarization

Group polarization is the strengthening of an original group attitude following the discussion of views within a group (Teger & Pruitt, 1967). That is, if a group initially favors a viewpoint, after discussion the group consensus is likely a stronger endorsement of the viewpoint. Conversely, if the group was initially opposed to a viewpoint, group discussion would likely lead to stronger opposition.
39.2K