Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Defenses Against Pathogens and Herbivores02:26

Defenses Against Pathogens and Herbivores

29.7K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
29.7K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

2.9K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.9K
Control System Problem01:21

Control System Problem

443
In an open-loop system, such as a basic thermostat, the poles of the transfer function influence the system's response but do not determine its stability. However, when feedback is introduced to form a closed-loop system, such as an advanced thermostat that adjusts heating based on room temperature, stability is governed by the new poles of the closed-loop transfer function.
When forming a closed-loop system, issues can arise if the poles cross into the unstable region, leading to potential...
443
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.9K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.9K
Combinatorial Gene Control02:33

Combinatorial Gene Control

9.7K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.7K
Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches01:23

Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches

475
Biopharmaceutical studies constitute a vital field aiming to enhance drug delivery methods and refine therapeutic approaches, drawing upon diverse interdisciplinary knowledge. In research methodologies, the choice between controlled and non-controlled studies significantly influences the study's reliability and accuracy.
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
475

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The toxin-antitoxin complex Fic-1-AntF functions as a deAMPylase that regulates the activity of DNA gyrase.

mLife·2026
Same author

Shikonin Targets Dihydroorotase to Inhibit the Growth and Virulence of Phytopathogenic Fungi.

Journal of agricultural and food chemistry·2026
Same author

Cross-Linked Reticular Magnetic Beads Immobilizing Streptavidin for Fishing the Protein of Interest with High Sensitivity.

ACS applied materials & interfaces·2026
Same author

β-Caryophyllene Competes With NINJA/IAAs for TOPLESS Family Corepressors to Regulate Plant Physiological Activities.

Plant, cell & environment·2026
Same author

Combinatorial Targeting of Common Docking and ATP Binding Sites on Mps1 MAPK for Management of Pathogenic Fungi.

Journal of agricultural and food chemistry·2024
Same author

Ningnanmycin Activates Defense Systems against Potato Virus Y in <i>Nicotiana benthamiana</i>.

Journal of agricultural and food chemistry·2024

Related Experiment Video

Updated: Feb 13, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function
06:06

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

6.0K

Gluing rice E3 ligase and pathogen effector for disease control.

Yongqi Chang1,2, Lili Zhou1,2, Tongtong Zhu1,2

  • 1State Key Laboratory of Maize Bio-breeding, China Agricultural University, Beijing, 100193, China.

Journal of Integrative Plant Biology
|February 11, 2026
PubMed
Summary

A novel compound, B93, triggers the degradation of the rice blast effector AVR-PikC by rice E3 ligase APIP6. This interaction enhances plant resistance against the rice blast fungus.

More Related Videos

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
10:27

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Published on: December 5, 2019

9.4K
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

3.2K

Related Experiment Videos

Last Updated: Feb 13, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function
06:06

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

6.0K
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
10:27

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Published on: December 5, 2019

9.4K
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

3.2K

Area of Science:

  • Plant pathology
  • Molecular biology
  • Biochemistry

Background:

  • The rice blast fungus (Magnaporthe oryzae) poses a significant threat to global rice production.
  • Fungal effectors, such as AVR-PikC, are key virulence factors that manipulate host processes.
  • AVR-PikC interacts with rice proteins, potentially contributing to disease susceptibility.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying AVR-PikC function in rice.
  • To identify strategies for enhancing rice resistance against the blast fungus.
  • To explore the potential of small molecules in modulating plant-pathogen interactions.

Main Methods:

  • Yeast three-hybrid assays to detect protein-protein interactions.
  • Co-immunoprecipitation to confirm interactions in planta.
  • Ubiquitination assays to assess protein modification.
  • Degradation assays to monitor protein turnover.
  • Phenotypic analysis of rice plants treated with B93.

Main Results:

  • The rice protein HIPP19 was identified as a binding partner of AVR-PikC, potentially mediating susceptibility.
  • The compound B93 was found to induce an interaction between AVR-PikC and the rice E3 ligase APIP6.
  • This induced interaction led to the ubiquitination and subsequent degradation of AVR-PikC.
  • Degradation of AVR-PikC resulted in enhanced resistance of rice plants to the blast fungus.

Conclusions:

  • The AVR-PikC-HIPP19 interaction is a potential target for modulating rice susceptibility.
  • The small molecule B93 effectively targets AVR-PikC for degradation via the APIP6 E3 ligase.
  • This study presents a novel strategy for engineering durable resistance in rice against the blast pathogen.