Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

5.8K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.8K
Facilitated Transport01:19

Facilitated Transport

151.4K
The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
151.4K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

3.0K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
3.0K
Bacterial Transformation01:33

Bacterial Transformation

60.2K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
60.2K
Intrinsically Disordered Proteins02:18

Intrinsically Disordered Proteins

19.6K
Intrinsically disordered proteins are a group of proteins that do not fold into specific three-dimensional structures. Their structural flexibility allows them to complement ordered proteins to perform functions that are inaccessible to rigid structures. They are more common in eukaryotes than prokaryotes and may either be exclusively intrinsically disordered or hybrid proteins, consisting of a mix of ordered and disordered regions. The absence of a rigid structure in these proteins can be...
19.6K
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.8K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcription factor collaboration enables precise T cell state engineering.

bioRxiv : the preprint server for biology·2026
Same author

Identifying Inheritance Patterns of Allelic Imbalance, using Integrative Modeling and Bayesian Inference.

bioRxiv : the preprint server for biology·2026
Same author

Modeling gene regulatory perturbations via deep learning from high-throughput reporter assays.

bioRxiv : the preprint server for biology·2026
Same author

Structured Pooling Improves Detection of Rare Regulatory Mutations in Population-Scale Reporter Assays.

bioRxiv : the preprint server for biology·2026
Same author

Reprogramming of neuronal genome function and phenotype by astrocytes.

bioRxiv : the preprint server for biology·2026
Same author

Mismatch tolerance of a gRNA for CRISPR-based gene activation confers broad activity critical for cell reprogramming.

bioRxiv : the preprint server for biology·2026
Same journal

Interplay between oxygen redox and interfacial stability of Li-rich positive electrodes in sulfide-based all-solid-state batteries.

Nature communications·2026
Same journal

Breaking dependence on melanisation imparts diversity to a dogmatic invasion strategy of phytopathogenic fungi.

Nature communications·2026
Same journal

Hydroxyl-rich nanocavities on perovskite enable nearly barrierless intramolecular hydrogen transfer for nitrate electroreduction to ammonia.

Nature communications·2026
Same journal

Household mobility responses to weather extremes in Kyrgyzstan.

Nature communications·2026
Same journal

Autonomous Motion Vision with Tri-bulk-heterojunctioned Organic Adaptation Transistor.

Nature communications·2026
Same journal

Tissue-adhesive hydrogel optical fiber for peripheral optogenetic neuromodulation.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Feb 13, 2026

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
08:01

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Published on: December 15, 2015

7.9K

dHyperCas12a enables multiplexed CRISPRi screens.

Schuyler M Melore1,2,3,4, Christian D McRoberts Amador3,4,5, Marisa C Hamilton1,3,4

  • 1University Program in Genetics & Genomics, Duke University, Durham, NC, USA.

Nature Communications
|February 11, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a new CRISPR tool, dHyperLbCas12a, for precise control over gene and cis-regulatory element activity. This system enables highly multiplexed gene expression studies and manipulation in various cell types.

More Related Videos

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19
06:46

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

3.4K
A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
10:13

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

11.7K

Related Experiment Videos

Last Updated: Feb 13, 2026

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
08:01

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection

Published on: December 15, 2015

7.9K
A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19
06:46

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Published on: July 5, 2022

3.4K
A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
10:13

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

11.7K

Area of Science:

  • Molecular Biology
  • Genetics
  • Gene Regulation

Background:

  • Gene and cis-regulatory element (CRE) interactions are crucial for biological processes.
  • CRISPR screens enable gene/CRE activity assessment, but interaction analysis is limited.

Purpose of the Study:

  • To develop a system for efficient, highly multiplexed control of regulatory element activity.
  • To enable dissection of independent and combinatorial contributions of CREs to gene expression.

Main Methods:

  • Combined a hyper-efficient dCas12a (dHyperLbCas12a) with long CRISPR RNA (crRNA) arrays.
  • Utilized RNA Polymerase II for expression of crRNA arrays.
  • Applied dHyperLbCas12a for simultaneous activation and repression.

Main Results:

  • Demonstrated multiplexed control of gene expression using activation and repression domains.
  • Successfully applied the system in cultured primary immune cells and for iPSC differentiation.
  • Showcased the ability to dissect independent and combinatorial CRE contributions.

Conclusions:

  • The dHyperLbCas12a system offers efficient, highly multiplexed control of gene expression.
  • This technology expands possibilities for studying gene regulation in diverse biological systems.
  • Enables detailed analysis of cis-regulatory element functions and interactions.