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Updated: Feb 13, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
Comprehensive volatile profiling and metabolomics analysis of cowpea
Xiuhua He1, Yanchi Zhou1, Qiaozhu Wei1
1School of Food Science and Engineering, Hainan University, Haikou 570228, China.
None:
As a high-value legume crop, the chemical basis underlying flavor and nutritional quality differences among cowpea (Vigna unguiculata L. Walp., Vu) varieties remains poorly understood, limiting targeted quality improvement. This study integrated headspace gas chromatography-ion mobility spectrometry with ultra-high-performance liquid chromatography-tandem mass spectrometry to comprehensively analyze metabolites in 14 Chinese cowpea varieties. A total of 174 volatile organic compounds were identified, with esters, heterocyclic compounds, and ketones accounting for 51.15% of the total. Principal component analysis and fingerprint clustering revealed variety-specific distribution patterns of volatile components, with the Vu-1 variety showing significant enrichment in β-pinene and 3-pentanol. Metabolomic analysis further identified 392 differentially expressed metabolites, revealing that amino acid metabolism and flavonoid metabolism are the core pathways determining varietal differences. This study systematically elucidates the chemical characteristics of cowpea varieties from a metabolomic perspective, providing important evidence for quality improvement and high-value utilization of legume crops.
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