Related Experiment Video
Updated: Feb 13, 2026

Staining Proteins in Gels
Published on: July 8, 2008
Quality Comparison of Apios Starch Gel and Whole Apios Gel and Effect of Oil Addition on Retrogradation Inhibition in
Soo Hyun Kim1, Ju Hong Park2, Nami Joo1
1Department of Food and Nutrition Sookmyung Women's University Seoul Republic of Korea.
Abstract:
Apios americana is an underutilized legume rich in carbohydrates, proteins, and micronutrients, yet research on its potential as a gel-based food and its retrogradation behavior remains limited. This study aimed to compare the physicochemical and sensory properties of gels prepared from whole Apios and Apios starch, and to investigate the effect of oil addition on retrogradation inhibition in whole Apios gel. Texture profile analysis, syneresis rate measurement, sensory evaluation, Differential Scanning Calorimetry (DSC), and Fourier Transform Infrared Spectroscopy (FTIR) were conducted to analyze gel characteristics and structural changes. Whole Apios gel showed significantly lower syneresis and superior texture acceptability compared with starch gel, receiving the highest overall liking score. Increasing oil content (0%-6%) decreased hardness, improved elasticity, and effectively reduced syneresis. DSC results indicated the lowest enthalpy value at 4% oil addition, demonstrating optimal retrogradation inhibition. FTIR analysis confirmed reduced hydrogen bonding and recrystallization, supporting the stabilizing mechanism of oil incorporation. These findings highlight the functional potential of whole Apios gel as a retrogradation-resistant gel product and suggest practical applications for value-added product development utilizing underused crops in the food industry.
Related Concept Videos
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Feedback Inhibition
Conjugate Addition (1,4-Addition) vs Direct Addition (1,2-Addition)
Conjugate addition results in a thermodynamically stable product. The reaction retains the stronger C=O bond at the expense of the weaker C=C π bond. The process is slow as the β carbon is less electrophilic than the carbonyl carbon.
Direct addition products are...
The Sense of Self: Reflected Self-Appraisal and Social Comparison

