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Assaying Proteasomal Degradation in a Cell-free System in Plants
Published on: March 26, 2014
Comprehensive Degradation Analysis of Swertiamarin: Stability-Indicating Assay and Structure Elucidation of
Bhakti Nanaware1, Pranav Shevkar1, Venkata Gopal Ede1
1Department of Natural Products, National Institute of Pharmaceutical Education and Research, Ahmedabad, Gandhinagar, Gujarat, India.
Introduction:
Swertiamarin (SWE) is a bioactive secoiridoid glycoside derived from the medicinal plant Enicostemma littorale and is a promising candidate for anti-diabetic therapy. However, SWE have shown poor bioavailability and stability concerns.
Objective:
This study aims to systematically investigate the stability of swertiamarin under controlled stress conditions according to International Council for Harmonisation (ICH) guidelines.
Materials And Methods:
A stability-indicating high-performance liquid chromatography (HPLC) method was developed and validated. Forced degradation of SWE was performed under heat (60°C), oxidative, and hydrolytic (acidic, basic, neutral) stress conditions.
Results:
SWE underwent rapid degradation under hydrolytic stress, degrading completely within 20 minutes in base and within 2 hours in acid. These stress conditions produced four degradation products namely 5‑hydroxysecologanolic acid, loganic acid, sweritranslactone C, and sweritranslactone B. Overall, SWE was stable at 60°C and significantly degraded under other tested stress conditions such as acidic, basic, aqueous neutral and oxidative conditions.
Conclusion:
The findings highlight the instability of SWE under stress environments and underscore the necessity of protective formulation strategies and controlled storage conditions to ensure its pharmaceutical efficacy in therapeutic applications.
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