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Updated: Feb 14, 2026

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization
Published on: September 2, 2022
Modifying pea protein via dynamic high-pressure microfluidization and polyphenol/polysaccharide incorporation to
Leichao Dong1, Guihua Sheng1, Yajie Li1
1School of Agricultural Engineering and Food Science, Shandong University of Technology, 255049 Zibo, China.
Abstract:
In this study, pea protein was modified by dynamic high-pressure microfluidization combined with chlorogenic acid and cellulose nanofiber incorporation to synthesize three ternary complexes for stabilizing 3D-printed high-internal-phase Pickering emulsions. DCC12 (pea protein:cellulose nanofiber:chlorogenic acid mass ratio = 1000:20:3) showed the smallest D50 size (4.7 ± 0.2 μm). Cellulose nanofibers maintain the zeta potential of DCC12 at -13.1 ± 0.2 mV; in their absence the zeta potential is reduced to -6.4 ± 0.2 mV. Besides, DCC12 showed good wettability (51.8 ± 1.6°), the highest free sulfhydryl content (1.9 ± 0.1 μmol/g), and the highest antioxidant capacity increase compared with the raw protein (DPPH/ABTS radical scavenging activity: 202.24%/66.16%). The DCC12-based gels exhibited outstanding thermal and centrifugal stabilities owing to interface layer enhancement by polyphenols. Therefore, DCC12 could be used to build gels with dense and stable networks and rheological and textural properties suggesting printability. These findings provide a strategy for the application of pea proteins in novel 3D-printed foods.
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